Transplanted GFP-expressing Brain Progenitor/Stem Cells (BPCs)Brain progenitor/stem cells can integrate and differentiate following transplantation into the mammalian retina. This figure illustrates green fluorescent protein expressing brain progenitor cells 28 days after transplantation into the eye of a Brazilian opossum, Monodelphis domestica, host. This cross-section of retinal tissue was stained with an antibody against calretinin, a marker used to identify subclasses of retinal neurons. To visualize the calretinin labeling pattern, we used Biotin-SP-Donkey Anti-Rabbit IgG (H+L) followed by Cy3-streptavidin. This image was created by merging confocal images of GFP fluorescence (green) with the calretinin antibody labeling pattern (red). Co-localization of the calretinin (red) in transplanted GFP-expressing progenitor cells (green) is indicated by the yellow color. Transplanted cells were located among calretinin-immunoreactive amacrine and ganglion cells. Jackson ImmunoResearch reagents used in this image: Biotin-SP-Donkey Anti-Rabbit IgG (H+L) (711-065-152) and Cy3-Streptavidin (016-160-084) Image contributed by Van Hoffelen, S.J., Young, M.J., Shatos, M., and Sakaguchi , D.S. (2003) Incorporation of murine brain progenitor cells into the developing mammalian retina. Investigative Ophthal. Visual Sci. 44:426-434. (This image is a modified version of that published in the article). back to photo menu |
