ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals.
The purified immunoglobulins in this section therefore do not represent antibodies directed against any known antigens.
ChromPure proteins are prepared by a variety of methods, including ion-exchange, gel-filtration, hydrophobic, dye-ligand,
metal-affinity, Protein A, and immunoaffinity chromatographies. F(ab')2 (pepsin) and Fab and Fc (papain) (see Figure
1) fragments are purified from enzyme digests of
highly purified whole molecules. No contaminating whole molecules or undesired fragments are observed at a minimum
protein concentration of 20 mg/ml when tested by immunoelectrophoresis against anti-whole serums, anti-immmunoglobulins
(class-specific), or anti-fragment-specific antisera. However, these proteins are not guaranteed to produce monospecific
antibodies when used for immunization. They are used primarily as experimental controls.
All unconjugated ChromPure proteins are sterile filtered in phosphate buffer without preservatives or stabilizers. Conjugated ChromPure proteins are freeze-dried in phosphate buffer with stabilizers and sodium azide, with the exception of horseradish peroxidase conjugates, which do not contain a preservative.
