Technical Center

Technical Information on  Probes Conjugated
to Our Antibodies and Other Proteins:

Fluorophores
Technical Service e-mail
tech@jacksonimmuno.com
The selection of fluorophores depends on:
  1. Instrument set-up. For example, availability of light sources, filter sets, and detection systems.
  2. Degree of color separation desired for multiple labeling. For example, Rhodamine Red-X (RRX) and Texas Red (TR) give better separation from fluorescein than tetramethyl rhodamine (TRITC).
  3. Sensitivity required. For example, Cy3 and Cy5 are brighter than other fluorophores.
• Aminomethylcoumarin Acetate (AMCA)

• Fluorescein Isothiocyanate (FITC)

• Cyanine dyes (Cy2, Cy3, and Cy5)

• Tetramethyl Rhodamine Isothiocyanate (TRITC), Rhodamine Red-X,
  and Texas Red (TR)

• NEW  Text Files for all fluorescent dye conjugates to compare excitation
  and emission spectra

Other Probes

• Biotin-SP (long-spacer)

• Enzymes

 
DyLight™
    
            NEW Fluorescent Dyes
A new family of fluorescent dyes with improved brightness and photostability
conjugated to Secondary Antibodies with recognized highest quality
and diversity from Jackson ImmunoResearch Laboratories, Inc
 
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