Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Cy™2 IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific

(min X Bov, Gt, Ar Hms, Hrs, Hu, Ms, Rat, Shp Ig)


Target: Rabbit
Host: Mouse
Antibody Format: IgG Fraction Monoclonal
Specificity: IgG, light chain specific
Minimal Cross Reactivity: Bovine, Goat, Armenian Hamster, Horse, Human, Mouse, Rat, Sheep Immunoglobulins
Conjugate: Cyanine Cy™2
Product Category: Antibody Conjugates With Limited Stock
Clonality: Monoclonal
RRID: AB_2339159

Discontinued - Limited Stock Available

Code: 211-222-171

Unit:
0.5 mg
Price:
$190.00



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Technical Info
Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on rabbit IgG and with those common to other rabbit immunoglobulins. The antibody does not react with the heavy chain of rabbit IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, armenian hamster, horse, human, mouse, rat and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Cyanine Cy™2

Amax: 492 Emax: 510nm

Cy2 conjugates have maximum absorption/excitation at 492 nm and fluoresce with a peak around 510 nm in the green region of the visible spectrum like FITC conjugates (520 nm), but they are more photostable and less sensitive to pH changes than FITC. However, for mounting in aqueous media we recommend Alexa Fluor® 488 as the preferred green-fluorescing dye because it is brighter and more photostable than Cy2, FITC, and DyLight 488. A further disadvantage of using Cy2 with aqueous mounting media is its sensitivity to p-phenylenediamine, an anti-fading agent found in some commercial mounting media, which results in weak and diffused fluorescence after storage of stained slides. The main advantage of Cy2 conjugates is increased fluorescence in plastic mounting media.

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Cy is a trademark of GE Healthcare Bio-Sciences Ltd. Jackson ImmunoResearch is licensed by GE Healthcare Bio-Sciences Ltd. to manufacture and sell conjugates of Cy2, Cy3, and Cy5 reactive dyes under US Patent Number 5,268,486, and other patents pending.

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