Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

AffiniPure™ Fab Fragment Rabbit Anti-Goat IgG (H+L)


Target: Goat
Host: Rabbit
Antibody Format: Fab Fragment
Specificity: IgG (H+L)
Conjugate: Unconjugated
Product Category: Fab Fragment Affinity‑Purified Antibodies
Clonality: Polyclonal
RRID: AB_2339388

Warning: Bovine serum albumin (BSA) and dry milk may contain IgG. Secondary antibodies directed against alpaca, bovine, goat, horse, or sheep react with bovine IgG. Therefore, use of BSA or milk to block or dilute these antibodies may significantly increase background and/or reduce antibody titer. For blocking, normal serum (5% v/v) from the host species of the labeled secondary antibody is recommended.

Code: 305-007-003

Unit:
1.0 mg
Price:
$120.00


Download GHS/SDS

Technical Info
Based on antigen-binding assay and/or ELISA, the antibody reacts with whole molecule goat IgG. It also reacts with the light chains of other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.

Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.
Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: None

Suggested Working Concentration or Dilution Range:

20-40 µg / ml

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
Jackson ImmunoResearch Laboratories, Inc. has trademark rights to AffiniPure™.

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