Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

AffiniPure™ Rabbit Anti-Sheep IgG (H+L)

(min X Hu Sr Prot)


Target: Sheep
Host: Rabbit
Antibody Format: Whole IgG
Specificity: IgG (H+L)
Minimal Cross Reactivity: Human Serum Proteins
Conjugate: Unconjugated
Product Category: Whole IgG Affinity-Purified Antibodies
Clonality: Polyclonal
RRID: AB_2339938

Warning: Bovine serum albumin (BSA) and dry milk may contain IgG. Secondary antibodies directed against alpaca, bovine, goat, horse, or sheep react with bovine IgG. Therefore, use of BSA or milk to block or dilute these antibodies may significantly increase background and/or reduce antibody titer. For blocking, normal serum (5% v/v) from the host species of the labeled secondary antibody is recommended.

Code: 313-005-045

Unit:
1.5 mg
Price:
$102.00


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Technical Info
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule sheep IgG. It also reacts with the light chains of other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.
Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: None

Suggested Working Concentration or Dilution Range:

10-20 µg / ml

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
Jackson ImmunoResearch Laboratories, Inc. has trademark rights to AffiniPure™.

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