Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

Cy™3 AffiniPure™ Donkey Anti-Goat IgG (H+L)

(min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)


Target: Goat
Host: Donkey
Antibody Format: Whole IgG
Specificity: IgG (H+L)
Minimal Cross Reactivity: Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat Serum Proteins
Conjugate: Cyanine Cy™3
Product Category: Whole IgG Affinity-Purified Antibodies
Clonality: Polyclonal
RRID: AB_2307351

Suitable for multiple labeling (see our Multiple Labeling guidance)
Warning: Bovine serum albumin (BSA) and dry milk may contain IgG. Secondary antibodies directed against alpaca, bovine, goat, horse, or sheep react with bovine IgG. Therefore, use of BSA or milk to block or dilute these antibodies may significantly increase background and/or reduce antibody titer. For blocking, normal serum (5% v/v) from the host species of the labeled secondary antibody is recommended.

Code: 705-165-147

Unit:
0.5 mg
Price:
$150.00


Download GHS/SDS

Technical Info
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule goat IgG. It also reacts with the light chains of other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, syrian hamster, horse, human, mouse, rabbit and rat serum proteins, but it may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

1:100 - 1:800 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Cyanine Cy™3

Amax: 550 Emax: 570nm

Cy3 is brighter, more photostable, and gives less background than other orange-red fluorescing dye conjugates. Cy3 conjugates can be excited maximally at 550 nm, with peak emission at 570 nm. For fluorescence microscopy, Cy3 can be visualized with traditional tetramethyl rhodamine (TRITC) filter sets, since the excitation and emission spectra are nearly identical to those of TRITC. We recommend Cy3 as a brighter alternative to TRITC. Cy3 can be excited to about 50% of maximum with an argon laser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser (543 nm line) or mercury lamp (546 nm line). Cy3 has been used with fluorescein for double labeling; however, the use of a narrow band-pass emission filter for fluorescein is recommended to minimize Cy3 fluorescence in the FITC filter set. Cy3 can also be paired with Alexa Fluor® 647 for multiple labeling when using a confocal microscope. However, a better choice for multiple labeling is Rhodamine Red-X because its fluorescence is midway between a green fluorescing dye (like Alexa Fluor® 488) and a far-red-fluorescing dye like Alexa Fluor® 647.


Above:

Two-week old mouse embryo showing neurons in green (stained with beta-III tubulin), endothelial cells in red (endomucin), and muscle in white (alpha-smooth muscle actin). Nuclei are blue (counterstained with DAPI). Jackson ImmunoResearch products: Alexa Fluor® 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) (min X) (703-545-155), Cy™3 AffiniPure Donkey Anti-Goat IgG (H+L) (min X) (705-165-147), and Alexa Fluor® 647 AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X) (711-605-152).

Below:

Lymphatic endothelial cells in culture. Cell-cell adhesion proteins stained red (using VEcadherin) and F-actin in cyan (phalloidin). Nuclei are yellow (counterstained with DAPI). Jackson ImmunoResearch products: Cy™3 AffiniPure Donkey Anti-Goat IgG (H+L) (min X) (705-165-147).

Credit: Derek Sung


This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
Jackson ImmunoResearch Laboratories, Inc. has trademark rights to AffiniPure™.

Cy is a trademark of GE Healthcare Bio-Sciences Ltd. Jackson ImmunoResearch is licensed by GE Healthcare Bio-Sciences Ltd. to manufacture and sell conjugates of Cy2, Cy3, and Cy5 reactive dyes under US Patent Number 5,268,486, and other patents pending.

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