Tissue specimen of 9 month old (middle-aged) Wistar rat retina illustrates connexin expression in astrocytes in the mid-peripheral retina.<\/p>\n
Six-marker immunohistochemistry was executed on retinal wholemount preparations. Connexin 26 (blue, Alexa Fluor\u00ae<\/sup> 532-conjugated anti-goat IgG), Connexin 30 (red, Cy3-conjugated anti-rabbit IgG), Connexin 43 (green, Alexa Fluor\u00ae<\/sup> 488-conjugated mouse IgG1), Connexin 45 (pale brown, Alexa Fluor\u00ae<\/sup> 594-conjugated anti-sheep IgG), GFAP stained for astrocytes (pale purple, Cy5-conjugated anti-chicken IgY), GS Isolectin B4 stained for blood vessels (turquoise, AMCA-conjugated streptavidin). Equipment used: Tissue specimen of 9 month old (middle-aged) Wistar rat retina illustrates connexin expression in astrocytes in the mid-peripheral retina. Six-marker immunohistochemistry was executed on retinal wholemount preparations. Connexin 26 (blue, Alexa Fluor\u00ae 532-conjugated anti-goat IgG), Connexin 30 (red, Cy3-conjugated anti-rabbit IgG), Connexin 43 (green, Alexa Fluor\u00ae 488-conjugated mouse IgG1), Connexin 45 (pale brown, Alexa Fluor\u00ae […]<\/p>\n","protected":false},"author":3,"featured_media":1262,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"jir-gallery-single-image-page.php","meta":{"content-type":""},"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1260"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=1260"}],"version-history":[{"count":11,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1260\/revisions"}],"predecessor-version":[{"id":1775,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1260\/revisions\/1775"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/1262"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=1260"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nCarl Zeiss LSM 510 META inverted confocal microscope equipped with UV-Argon laser scanning, Axiovert 200M and LSM 510 scan head. The objective lens used was a Plan-Apochromat 63x\/1.40 Oil DIC M27 and excitation laser lines (405, 488, 561 and 633 nm). Image captured of optical z-series projection, resolution of 1024 x 1024, and data depth of 12.<\/p>\n","protected":false},"excerpt":{"rendered":"