{"id":1355,"date":"2017-04-28T06:06:44","date_gmt":"2017-04-28T10:06:44","guid":{"rendered":"https:\/\/jacksonimmuno.com\/secondary-antibody-resource\/?page_id=1355"},"modified":"2017-04-28T06:22:28","modified_gmt":"2017-04-28T10:22:28","slug":"triple-ihc-gallery-image","status":"publish","type":"page","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/triple-ihc-gallery-image\/","title":{"rendered":"Triple IHC gallery image"},"content":{"rendered":"<p>Triple label IHC! Retinal wholemount preparations. GFAP stained for astrocytes. Vascular-associated astrocytes in the young adult rat retina<br \/>\nTissue specimen of 3 month old (young adult) Wistar rat retina illustrates vascular-associated astrocytes in the mid-peripheral retina. Triple label immunohistochemistry was executed on retinal wholemount preparations. GFAP stained for astrocytes (red, Cy3-conjugated mouse monoclonal anti-GFAP), GS Isolectin B4 stained for blood vessels (blue, Cy5-conjugated streptavidin), and S-100 stained for cell soma region of astrocytes (green, Alexa 488-conjugated anti-rabbit IgG). Equipment used:<br \/>\nCarl Zeiss LSM 510 META inverted confocal microscope equipped with UV-Argon laser scanning, Axiovert 200M and LSM 510 scan head. The objective lens used was a Plan-Apochromat 40x\/0.5. Alexa488 fluorescence was excited at 488 nm, Cy3 at 561 nm and Cy5 at 633 nm. Image captured of optical z-series projection, resolution of 1024 x 1024, and data depth of 12.<\/p>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Triple label IHC! Retinal wholemount preparations. GFAP stained for astrocytes. Vascular-associated astrocytes in the young adult rat retina Tissue specimen of 3 month old (young adult) Wistar rat retina illustrates vascular-associated astrocytes in the mid-peripheral retina. Triple label immunohistochemistry was executed on retinal wholemount preparations. GFAP stained for astrocytes (red, Cy3-conjugated mouse monoclonal anti-GFAP), GS [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":3,"featured_media":1360,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"jir-gallery-single-image-page.php","meta":{"content-type":""},"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1355"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=1355"}],"version-history":[{"count":4,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1355\/revisions"}],"predecessor-version":[{"id":1861,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1355\/revisions\/1861"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/1360"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=1355"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}