{"id":1396,"date":"2017-04-28T06:56:15","date_gmt":"2017-04-28T10:56:15","guid":{"rendered":"https:\/\/jacksonimmuno.com\/secondary-antibody-resource\/?page_id=1396"},"modified":"2017-04-28T06:56:15","modified_gmt":"2017-04-28T10:56:15","slug":"human-lymph-node","status":"publish","type":"page","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/human-lymph-node\/","title":{"rendered":"Human Lymph Node"},"content":{"rendered":"<p>Human lymph node<br \/>\n2um thick human lymph node, serial sections<br \/>\nPrimary Abs: overnight @4C<br \/>\nMouse @CD20-eFluor660 1:50<br \/>\nRabbit @CD3 1:200<br \/>\nSecondary Ab: 1h RT, Donkey anti &#8211; rabbit IgG-PerCP (711-126-152),<br \/>\nImage acquisition:<br \/>\nLeica Upright SP8 confocal microscope.<br \/>\nPerCP excitation: 488nm laser intensity 3.4%, detection by HyD 144%<br \/>\neFluor660 excitation: 638nm laser intensity 0.4%, detection by HyD 32%<br \/>\nDAPI excitation: 405nm laser intesity 0.2%, detection by HyD 50%<\/p>\n<p>&nbsp;<\/p>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Human lymph node 2um thick human lymph node, serial sections Primary Abs: overnight @4C Mouse @CD20-eFluor660 1:50 Rabbit @CD3 1:200 Secondary Ab: 1h RT, Donkey anti &#8211; rabbit IgG-PerCP (711-126-152), Image acquisition: Leica Upright SP8 confocal microscope. PerCP excitation: 488nm laser intensity 3.4%, detection by HyD 144% eFluor660 excitation: 638nm laser intensity 0.4%, detection by [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":3,"featured_media":1397,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"jir-gallery-single-image-page.php","meta":{"content-type":""},"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1396"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=1396"}],"version-history":[{"count":4,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1396\/revisions"}],"predecessor-version":[{"id":1875,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1396\/revisions\/1875"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/1397"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=1396"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}