{"id":1399,"date":"2017-04-28T06:57:17","date_gmt":"2017-04-28T10:57:17","guid":{"rendered":"https:\/\/jacksonimmuno.com\/secondary-antibody-resource\/?page_id=1399"},"modified":"2017-04-28T06:57:17","modified_gmt":"2017-04-28T10:57:17","slug":"expression-gfp-by-anti-egfp","status":"publish","type":"page","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/expression-gfp-by-anti-egfp\/","title":{"rendered":"Expression of GFP by anti-EGFP"},"content":{"rendered":"<p>Retrograde labelling of sub-coeruleus neurones using an adenoviral vector.<br \/>\nAn adenoviral vector containing PRSx8-Kir2.1-IRES-EGFP was injected into the lumbar dorsal horn of the rat. Retrograde labelling is observed in pontospinal regions (A5-A7). Although EGFP expression is sparse (1), it is possible to detect expression by using anti-EGFP (see 2, 1:4000, A11122, Invitrogen, 1:1000, Biotin SP anti-rabbit, 711-066-152, Jackson ImmunoResearch and Streptavidin Cy3, 1:1000, Sigma). These retrogradely labelled neurones were D\u03b2H positive (see 3 , 1:5000, MAB308, chemicon, 1:200, AMCA conjugated anti-mouse, 715-156-150, Jackson ImmunoResearch). Key: 4 &#8211; overlay; scale bars 50\u03bcM.<\/p>\n<p>&nbsp;<\/p>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Retrograde labelling of sub-coeruleus neurones using an adenoviral vector. An adenoviral vector containing PRSx8-Kir2.1-IRES-EGFP was injected into the lumbar dorsal horn of the rat. Retrograde labelling is observed in pontospinal regions (A5-A7). Although EGFP expression is sparse (1), it is possible to detect expression by using anti-EGFP (see 2, 1:4000, A11122, Invitrogen, 1:1000, Biotin SP [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":3,"featured_media":1401,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"jir-gallery-single-image-page.php","meta":{"content-type":""},"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1399"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=1399"}],"version-history":[{"count":6,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1399\/revisions"}],"predecessor-version":[{"id":1876,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1399\/revisions\/1876"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/1401"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=1399"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}