{"id":1508,"date":"2017-04-28T06:54:38","date_gmt":"2017-04-28T10:54:38","guid":{"rendered":"https:\/\/jacksonimmuno.com\/secondary-antibody-resource\/?page_id=1508"},"modified":"2017-04-28T06:54:38","modified_gmt":"2017-04-28T10:54:38","slug":"sub-coeruleus-neurones","status":"publish","type":"page","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/sub-coeruleus-neurones\/","title":{"rendered":"Localization of MUC16 protein"},"content":{"rendered":"<p>Retrograde labelling of sub-coeruleus neurones using an adenoviral vector.<br \/>\nAn adenoviral vector containing PRSx8-Kir2.1-IRES-EGFP was injected into the lumbar dorsal horn of the rat. Retrograde labelling is observed in pontospinal regions (A5-A7). Although EGFP expression is sparse, it is possible to detect expression by using anti-EGFP (RED, 1:4000, A11122, Invitrogen, 1:1000, Biotin SP anti-rabbit, 711-066-152, Jackson ImmunoResearch and strept-avidin Cy3, 1:1000, Sigma). These retrogradely labelled neurones were D\u03b2H positive (BLUE , 1:5000, MAB308, chemicon, 1:200, AMCA conjugated anti-mouse, 715-156-150, Jackson ImmunoResearch). Key: 4 &#8211; overlay; scale bars 50\u03bcM.<\/p>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Retrograde labelling of sub-coeruleus neurones using an adenoviral vector. An adenoviral vector containing PRSx8-Kir2.1-IRES-EGFP was injected into the lumbar dorsal horn of the rat. Retrograde labelling is observed in pontospinal regions (A5-A7). Although EGFP expression is sparse, it is possible to detect expression by using anti-EGFP (RED, 1:4000, A11122, Invitrogen, 1:1000, Biotin SP anti-rabbit, 711-066-152, [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":3,"featured_media":1509,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"jir-gallery-single-image-page.php","meta":{"content-type":""},"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1508"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=1508"}],"version-history":[{"count":5,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1508\/revisions"}],"predecessor-version":[{"id":1873,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1508\/revisions\/1873"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/1509"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=1508"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}