{"id":1557,"date":"2017-04-28T05:35:31","date_gmt":"2017-04-28T09:35:31","guid":{"rendered":"https:\/\/jacksonimmuno.com\/secondary-antibody-resource\/?page_id=1557"},"modified":"2017-04-28T05:35:31","modified_gmt":"2017-04-28T09:35:31","slug":"hep-2-cells","status":"publish","type":"page","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/hep-2-cells\/","title":{"rendered":"Distribution of Promyelocytic (PML)"},"content":{"rendered":"<p>Hep-2 cells expressing transiently the adenovirus-5 E4-ORF3 protein were analyzed by indirect immunofluorescence using a primary PML monoclonal Ab. Distinct PML-stained cell preparations were labeled with a highly pre-absorbed donkey anti-mouse secondary antibody conjugated to FITC (Jackson ImmunoResearch, code number 715-095-150). Fluorescence images were analyzed in confocal microscopy using a confocal laser scan fluorescence-inverted microscope (LSM 410, Zeiss). Confocal images represent single optical sections. Image analysis was subsequently entered into Photoshop where images were processed and magnified. PML and PML-associated macromolecular complexes have been implicated in mediating transcriptional regulation, suppressing cell cycle progression, inducing apoptosis or cellular senescence, and regulating viral propagation.<\/p>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Hep-2 cells expressing transiently the adenovirus-5 E4-ORF3 protein were analyzed by indirect immunofluorescence using a primary PML monoclonal Ab. Distinct PML-stained cell preparations were labeled with a highly pre-absorbed donkey anti-mouse secondary antibody conjugated to FITC (Jackson ImmunoResearch, code number 715-095-150). Fluorescence images were analyzed in confocal microscopy using a confocal laser scan fluorescence-inverted microscope [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":3,"featured_media":1558,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"jir-gallery-single-image-page.php","meta":{"content-type":""},"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1557"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=1557"}],"version-history":[{"count":6,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1557\/revisions"}],"predecessor-version":[{"id":1847,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/pages\/1557\/revisions\/1847"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/1558"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=1557"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}