{"id":10173,"date":"2023-05-19T10:50:30","date_gmt":"2023-05-19T14:50:30","guid":{"rendered":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/?p=10173"},"modified":"2024-10-31T09:01:22","modified_gmt":"2024-10-31T13:01:22","slug":"webinar-nanobodies-for-super-resolution-microscopy-3","status":"publish","type":"post","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/company-news\/webinar-nanobodies-for-super-resolution-microscopy-3\/","title":{"rendered":"NANOBODIES\u00ae for Super-Resolution Microscopy: Partnership between Jackson ImmunoResearch and Abberior."},"content":{"rendered":"\n\n\n\t<div class=\"dkpdf-button-container\" style=\" text-align:right \">\n\n\t\t<a class=\"dkpdf-button\" href=\"\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/10173?pdf=10173\" target=\"_blank\"><span class=\"dkpdf-button-icon\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span> Download PDF<\/a>\n\n\t<\/div>\n\n\n\n\n\n<style>.entry p, .entry ol{font-size: 1rem;}.entry h3{color:#009fe3;margin-top:0}.entry h4{color:#009453;margin-top:1.5rem}.entry h5{color: #003a5c; font-size: 1.05rem;margin-top:1.5rem;}.entry figure{margin:32px auto;border:1px solid #ccc}.entry figure img{width:100%;display:block;margin:0 auto}.entry figcaption{font-size:.875rem;line-height:1.35rem;padding:10px;color:#222}.entry .blog-tbl{margin:1rem auto;caption-side:bottom}.entry .blog-tbl td,.entry .blog-tbl th{padding:10px 9px;border:1px solid #00172b;text-align:left}.entry .blog-tbl td{border-color:#003a5c}.entry .blog-tbl th{background-color:#00172b;color:#fff;border-left-color:#fff;border-right-color:#fff}.entry .blog-tbl th p{color:#fff}.entry .blog-tbl th:first-of-type{border-left-color:#00172b}.entry .blog-tbl th:last-of-type{border-right-color:#00172b}.entry .blog-tbl p{text-align:left;margin:0}.entry .box-note{border:2px solid #009453;padding:12px;margin:1rem 0}.entry .box-note p{margin:0;padding:0}.entry .styled-list{list-style-type:none}.entry .styled-list li{margin-top:1rem;line-height:1rem;font-size:1rem;}.entry .styled-list li::before{font-family:\"Font Awesome 5 Pro\";display:inline-block;content:\"\\f3c5\";-webkit-transform:rotate(-90deg);transform:rotate(-90deg);margin-left:-20px;margin-right:11px;font-size:.75rem;color:#ed7004;font-weight:600}.entry .styled-list ol li::before{display:none}.entry .styled-list li>ul li::before{font-weight:200}.entry .overview{width:-webkit-fit-content;width:-moz-fit-content;width:fit-content;padding:16px;margin:1rem auto;border:1px solid #eee}.entry .overview hr{margin-top:14px}.entry .overview-text{text-align:center;font-size:1rem;margin:0}.entry .btn-sq{color:#fff;font-size:.9rem;font-weight:600;border:2px solid rgb(237, 112, 4);padding:7px 13px;background:rgb(237, 112, 4);cursor:pointer;text-align:center;line-height:1.5rem;margin:0 auto;}.entry .btn-sq:hover{text-decoration:none;color:rgb(237, 112, 4);background:#fff;}.entry .btn-container{display:flex;width:100%;margin:1.3rem 0;}.entry .btn-container .fa-solid, .entry .btn-container .fa-duotone{margin-right:10px;}@media(max-width: 768px){.entry .btn-sq{font-size:1.05rem;}}<\/style>\n<style>.entry figure img {width:95%;margin:8px auto;}.entry .styled-list li {font-weight: 600;}<\/style>\n<p><script src=\"https:\/\/kit.fontawesome.com\/904923013f.js\" crossorigin=\"anonymous\"><\/script><\/p>\n<h2>Looking to enhance your achievable resolution for STED and SRM? Combining small polyclonal AffiniPure-VHH\u2122 antibodies from Jackson ImmunoResearch and super bright STAR dyes from Abberior allows you to achieve the detail needed for high-impact results. Learn more about super-resolution imaging with JIR and Abberior\u2026<\/h2>\n<p><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/company-news\/webinar-nanobodies-for-super-resolution-microscopy\/\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-10317 size-full\" style=\"width: 100%; display: block;\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Abberior-Webinar-slider-post.jpg\" alt=\"\" width=\"707\" height=\"404\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Abberior-Webinar-slider-post.jpg 707w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Abberior-Webinar-slider-post-300x171.jpg 300w\" sizes=\"(max-width: 707px) 100vw, 707px\" \/><\/a><\/p>\n<p><span style=\"font-weight: 400;\">Jackson ImmunoResearch and Abberior have combined expertise to offer the novel format AffiniPure-VHH\u2122 Secondary Antibody conjugated to Abberior\u2019s super bright STAR dyes. The VHH (nanobody) format, as seen in Figure 1, is a small heavy-chain-only antibody fragment that offers an exciting new opportunity to improve resolution by reducing the linkage error between the target antigen and the dye conjugate (Figure 2 A).\u00a0<\/span><\/p>\n<figure><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-10246\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/vhh-cartoon-scaled.jpg\" alt=\"\" width=\"600\" height=\"232\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/vhh-cartoon-scaled.jpg 2560w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/vhh-cartoon-300x116.jpg 300w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/vhh-cartoon-1024x397.jpg 1024w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/vhh-cartoon-1536x595.jpg 1536w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/vhh-cartoon-2048x793.jpg 2048w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><figcaption><strong>Figure 1:<\/strong> VHH fragments from Camelid heavy chain-only antibodies are much smaller than conventional IgGs.<\/figcaption><\/figure>\n<p><span style=\"font-weight: 400;\">Indirect immunostaining with polyclonal secondary reagents offers optimal signal generation due to the amplification created through the binding of multiple secondary reagents heterogeneously across the primary antibody, but linkage error can preclude their use in super-resolution microscopy (SRM) techniques (Figure 3 C).\u00a0<\/span><\/p>\n<figure><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-10247\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/JIR-AffiniPure-VHH\u2122-poly-mono-Illustration.jpg\" alt=\"\" width=\"600\" height=\"318\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/JIR-AffiniPure-VHH\u2122-poly-mono-Illustration.jpg 936w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/JIR-AffiniPure-VHH\u2122-poly-mono-Illustration-300x159.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><figcaption><strong>Figure 2:<\/strong> Polyclonal antibodies offer ultimate conjugate delivery for maximum signal: Schematic showing the increased number of conjugated polyclonal AffiniPure-VHH\u2122 Secondaries able to bind a primary antibody compared to monoclonal nanobodies. Also, it can be seen that the diameter of the complex remains comparable, with the distance between the probe and target protein being similar.<\/p>\n<\/figcaption><\/figure>\n<figure><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-10300\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/VHH-Artboard-1-100.jpg\" alt=\"\" width=\"600\" height=\"277\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/VHH-Artboard-1-100.jpg 911w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/VHH-Artboard-1-100-300x138.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><figcaption><strong>Figure 3: <\/strong>Reduced linkage error of indirect staining using\u00a0 IgG+AffiniPure-VHH\u2122 Secondary Antibody compared to indirect staining using conventional whole IgG primary and secondary antibodies.<\/figcaption><\/figure>\n<p>At 15 kDa, <b>AffiniPure-VHH\u2122 polyclonal antibodies<\/b><span style=\"font-weight: 400;\"> offer excellent<\/span><b> tissue penetration<\/b><span style=\"font-weight: 400;\"> with <\/span><b>superior conjugate delivery<\/b><span style=\"font-weight: 400;\"> and <\/span><b>decrease the linkage error<\/b><span style=\"font-weight: 400;\"> considerably (Figure 3 B), bringing the conjugate much closer to the target antigen when compared to the conventional indirect immunostaining with whole IgGs. AffiniPure-VHH\u2122 antibodies are <\/span><b>cross-adsorbed<\/b><span style=\"font-weight: 400;\"> for exquisite specificity against target species with minimal cross-reactivity to other commonly used species, making AffiniPure VHH\u2122 format an excellent choice for high-resolution multiple labeling experiments. (Figures 4 and 5)<\/span><\/p>\n<div class=\"btn-container\"><a class=\"btn-sq\" href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/AffiniPure-VHH-secondary-antibodies\"><i class=\"fa-solid fa-book-open-reader\"><\/i>Read more about Jackson ImmunoResearch AffiniPure-VHH\u2122<\/a><\/div>\n<p><span style=\"font-weight: 400;\">Although JIR AffiniPure VHH\u2122 are available conjugated to a wide range of fluorescent dye conjugates, including AlexaFluor\u00ae, SRM techniques such as STED (Stimulated Emission Depletion Microscopy) and STORM (Stochastic Optical Reconstruction Microscopy) need dyes with specialized spectral characteristics and behaviors. Abberior is well known for the superior chemistry of its dyes and their commitment to providing exceptionally bright dyes for SRM imaging.\u00a0<\/span><\/p>\n<hr \/>\n<h3>The brightest dyes for SRM: Abberior STAR dyes<\/h3>\n<p><span style=\"font-weight: 400;\">Abberior STAR dyes have been designed and manufactured by Abberior\u2019s chemists for the best performance in STED and confocal microscopy. They offer high brightness and photostability, while the dye conjugates also show excellent solubility in aqueous buffers such as PBS, allowing for background-free labeling in cells or tissue.\u00a0<\/span><\/p>\n<figure><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-10253 size-full\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/IF2Artboard-5.png\" alt=\"\" width=\"612\" height=\"397\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/IF2Artboard-5.png 612w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/IF2Artboard-5-300x195.png 300w\" sizes=\"(max-width: 612px) 100vw, 612px\" \/><figcaption><strong>Figure 4:<\/strong> Single color microscopy. Indirect immunostaining was performed with JIR AffiniPure VHH\u2122 Anti-Mouse antibodies conjugated to Abberior STAR RED. Clear improvements in resolution can be seen when comparing confocal to STED microscopy.<\/figcaption><\/figure>\n<p><span style=\"font-weight: 400;\">Abberior offers\u00a0 <\/span><span style=\"font-weight: 400;\">AffiniPure-VHH\u2122 antibodies <\/span><span style=\"font-weight: 400;\">conjugated to three Abberior STAR dyes.\u00a0<\/span><\/p>\n<ul class=\"styled-list\">\n<li><i>abberior<\/i> STAR RED<\/li>\n<li><i>abberior<\/i> STAR 580<\/li>\n<li><i>abberior<\/i> STAR 460L<\/li>\n<\/ul>\n<div class=\"btn-container\"><a class=\"btn-sq\" href=\"https:\/\/abberior.shop\/abberior-STAR-\"><i class=\"fa-solid fa-graduation-cap\"><\/i>Learn more about Abberior STAR dyes<\/a><\/div>\n<p><span style=\"font-weight: 400;\">Available with specificity to Rabbit or Mouse, they are cross-adsorbed for exquisite specificity against target species with minimal cross-reactivity to other commonly used species, making them suitable for application in multiple labeling experiments.\u00a0<\/span><\/p>\n<figure><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-10252 size-full\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/IF2Artboard-4.png\" alt=\"\" width=\"612\" height=\"397\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/IF2Artboard-4.png 612w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/IF2Artboard-4-300x195.png 300w\" sizes=\"(max-width: 612px) 100vw, 612px\" \/><figcaption><strong>Figure 5:<\/strong> Two-color indirect immunostaining. The comparison of confocal microscopy to STED SRM highlights the high-resolution imaging achievable with JIR AffiniPure VHH\u2122 antibodies and Abberior STAR dyes.\u00a0 Imaging performed on the Abberior STEDYCON platform.<\/figcaption><\/figure>\n<p><span style=\"font-weight: 400;\">Register to watch our Webinar to learn more about JIR AffinPure VHH\u2122 antibodies and Abberior STAR dyes for super-resolution microscopy.<\/span><\/p>\n<p><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/AffiniPure-VHH-secondary-antibodies\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-9850 size-full\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Nano-Secondaries-Advert-ML-GIFFING-4.gif\" alt=\"\" width=\"600\" height=\"100\" \/><\/a><\/p>\n<\/p>\n<table class=\"table blogLinks\">\n<thead>\n<tr>\n<th>Learn more:<\/th>\n<th>Do more:<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/groups\/whole-igg\/Anti-Camelid\">Anti-Camelid\u00a0Secondary Antibodies<\/a><\/td>\n<td><a href=\"\/home\/exhibitions\">Exhibition schedule<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"\/technical\/products\/antibody-selection\">Choosing your secondary antibod<\/a>y<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td><a href=\"\/secondary-antibody-resource\/immuno-techniques\/colorimetric-western-blotting\/\">Colorimetric Western blotting<\/a><\/td>\n<td><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\n<p>NANOBODY\u00ae and NANOBODIES\u00ae are registered trademarks of Ablynx N.V.<\/p>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Download PDF Looking to enhance your achievable resolution for STED and SRM? Combining small polyclonal AffiniPure-VHH\u2122 antibodies from Jackson ImmunoResearch and super bright STAR dyes from Abberior allows you to achieve the detail needed for high-impact results. Learn more about super-resolution imaging with JIR and Abberior\u2026 Jackson ImmunoResearch and Abberior have combined expertise to offer [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":3,"featured_media":10241,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"content-type":""},"categories":[19,20],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/10173"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=10173"}],"version-history":[{"count":50,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/10173\/revisions"}],"predecessor-version":[{"id":11080,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/10173\/revisions\/11080"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/10241"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=10173"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/categories?post=10173"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/tags?post=10173"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}