{"id":10420,"date":"2023-08-07T06:57:37","date_gmt":"2023-08-07T10:57:37","guid":{"rendered":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/?p=10420"},"modified":"2023-08-07T07:01:10","modified_gmt":"2023-08-07T11:01:10","slug":"tissue-typing","status":"publish","type":"post","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/trending-topics\/tissue-typing\/","title":{"rendered":"Tissue Typing"},"content":{"rendered":"\n\n\n\t<div class=\"dkpdf-button-container\" style=\" text-align:right \">\n\n\t\t<a class=\"dkpdf-button\" href=\"\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/10420?pdf=10420\" target=\"_blank\"><span class=\"dkpdf-button-icon\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span> Download PDF<\/a>\n\n\t<\/div>\n\n\n\n\n\n<p><style>.entry p, .entry ol{font-size: 1rem;}.entry h3{color:#009fe3;margin-top:0}.entry h4{color:#009453;margin-top:1.5rem}.entry h5{color: #003a5c; font-size: 1.05rem;margin-top:1.5rem;}.entry figure{margin:32px auto;border:1px solid #ccc}.entry figure img{width:100%;display:block;margin:0 auto}.entry figcaption{font-size:.875rem;line-height:1.35rem;padding:10px;color:#222}.entry .blog-tbl{margin:1rem auto;caption-side:bottom}.entry .blog-tbl td,.entry .blog-tbl th{padding:10px 9px;border:1px solid #00172b;text-align:left}.entry .blog-tbl td{border-color:#003a5c}.entry .blog-tbl th{background-color:#00172b;color:#fff;border-left-color:#fff;border-right-color:#fff}.entry .blog-tbl th p{color:#fff}.entry .blog-tbl th:first-of-type{border-left-color:#00172b}.entry .blog-tbl th:last-of-type{border-right-color:#00172b}.entry .blog-tbl p{text-align:left;margin:0}.entry .box-note{border:2px solid #009453;padding:12px;margin:1rem 0}.entry .box-note p{margin:0;padding:0}.entry .styled-list{list-style-type:none}.entry .styled-list li{margin-top:1rem;line-height:1rem;font-size:1rem;}.entry .styled-list li::before{font-family:\"Font Awesome 5 Pro\";display:inline-block;content:\"\\f3c5\";-webkit-transform:rotate(-90deg);transform:rotate(-90deg);margin-left:-20px;margin-right:11px;font-size:.75rem;color:#ed7004;font-weight:600}.entry .styled-list ol li::before{display:none}.entry .styled-list li>ul li::before{font-weight:200}.entry .overview{width:-webkit-fit-content;width:-moz-fit-content;width:fit-content;padding:16px;margin:1rem auto;border:1px solid #eee}.entry .overview hr{margin-top:14px}.entry .overview-text{text-align:center;font-size:1rem;margin:0}.entry .btn-sq{color:#fff;font-size:.9rem;font-weight:600;border:2px solid rgb(237, 112, 4);padding:7px 13px;background:rgb(237, 112, 4);cursor:pointer;text-align:center;line-height:1.5rem;margin:0 auto;}.entry .btn-sq:hover{text-decoration:none;color:rgb(237, 112, 4);background:#fff;}.entry .btn-container{display:flex;width:100%;margin:1.3rem 0;}.entry .btn-container .fa-solid, .entry .btn-container .fa-duotone{margin-right:10px;}@media(max-width: 768px){.entry .btn-sq{font-size:1.05rem;}}<\/style> <style>.entry .styled-list li::before{display:none} .entry .styled-list li{list-style-type:decimal;line-height:24px}.entry .styled-list li::marker{font-size:75%}<\/style><\/p>\n<h2>Tissue typing, also known as human leukocyte antigen (HLA) testing, is the process of determining whether a donor tissue or organ will be compatible with its intended host. It involves comparing HLA expression between the prospective donor and the recipient to determine how similar the two antigen patterns are. The closer the match, the lower the risk of organ rejection and the greater the chances of long-term graft survival. At the same time that HLA expression is being analyzed, the recipient\u2019s blood is screened for pre-existing anti-HLA donor-specific antibodies (DSA) which could stimulate an adverse immune response against the transplanted material. Jackson ImmunoResearch Anti-Human IgG FITC Secondary Antibodies are widely used to test for DSA and are a recognized component of the flow cytometric crossmatch (FCXM) assay.<\/h2>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-10423 size-full\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/transplant-scene.jpg\" alt=\"\" width=\"700\" height=\"167\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/transplant-scene.jpg 700w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/transplant-scene-300x72.jpg 300w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><\/p>\n<h4><strong>What is the HLA system?<\/strong><\/h4>\n<p>The HLA system is the human version of the major histocompatibility complex (MHC), a large group of genes found on vertebrate DNA that encodes cell surface proteins with essential roles in regulating the immune response. It maps to the short arm of chromosome 6 (6p21) and is organized into three regions, class I, II, and III. Of these, class I and II serve to present processed peptide antigens to T cells, while class III encodes various immune regulatory molecules, many of which are important in inflammation. These include complement components C2, C4, and factor B, as well as various heat shock proteins and tumor necrosis factors<sup>1,2<\/sup>.<\/p>\n<p>Critically, the immune system uses HLAs to distinguish between \u2018self\u2019 and \u2018non-self\u2019. Any cells that display an individual\u2019s unique HLA type are recognized by the immune system as harmless, whereas those with a different HLA pattern to the host are seen as foreign invaders. In addition to being polygenic, the HLA system is highly polymorphic, and is currently known to comprise over 36,000 different alleles<sup>3<\/sup>. This makes it clinically important in the transplantation of foreign tissues and organs, where tissue typing represents a critical step in determining whether a transplant should go ahead.<\/p>\n<h4><strong>How is the HLA system used for tissue typing?<\/strong><\/h4>\n<p>Tissue typing has historically relied on serological methods. These involve mixing well-characterized anti-HLA antibodies (each with specificity for a single HLA) with lymphocytes from the individual being tissue typed. T cells are used for defining class I HLA molecules and B cells for defining class II HLA. When complement is added to the reaction, any cells with bound antibodies are lyzed, signifying the presence of that HLA on the cell surface<sup>4<\/sup>.<\/p>\n<p>A main advantage of serological tissue typing is that it is relatively quick and inexpensive to perform. However, serological tissue typing is unable to distinguish between different HLA sub-classes and does not permit full HLA characterization. Consequently, tissue typing methods based on genetic sequencing are seeing increased use. For example, next-generation sequencing (NGS) provides improved HLA genotype accuracy, although challenges remain for computation and data processing <sup>5<\/sup>.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-10425 size-full\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/dialysis.jpg\" alt=\"\" width=\"700\" height=\"175\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/dialysis.jpg 700w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/dialysis-300x75.jpg 300w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><\/p>\n<h4><strong>How is DSA testing performed?<\/strong><\/h4>\n<p>The original method for DSA testing, known as the complement-dependent cytotoxicity crossmatch (CDC-XM) assay, follows similar principles to serological tissue typing. However, instead of commercial anti-HLA antibodies, it uses the recipient\u2019s serum. When the serum is mixed with the potential donor cells, the presence of any anti-HLA DSA in the serum causes cell lysis when complement is added to the reaction<sup>6<\/sup>.<\/p>\n<p>Although CDC-XM is still used, its limited sensitivity has led to the development of alternative methods. One such approach is the flow cytometric crossmatch (FCXM) assay, during which the recipient\u2019s serum and enriched donor lymphocytes are mixed together before any bound antibodies are detected with a fluorescently-labeled F(ab\u2019)<sub>2<\/sub> specific to human IgG<sup>7<\/sup>. Critically, using a F(ab\u2019)<sub>2 <\/sub>eliminates the risk of antibody binding to bind to Fc receptors at the cell surface, which could generate false positive results. Jackson ImmunoResearch\u2019s Fluorescein (FITC) AffiniPure F(ab&#8217;)\u2082 Fragment Goat Anti-Human IgG, Fc\u03b3 fragment specific antibody (<a href=\"https:\/\/www.jacksonimmuno.com\/catalog\/products\/109-096-098\">109-096-098<\/a>) is widely used for FCXM, including for experiments that combine DSA detection with the identification of T and B cells using labeled anti-CD3 and anti-CD19 antibodies, respectively.<\/p>\n<p>To reduce assay times and simplify set-up, two modified versions of the FCXM were reported in 2017<sup>8<\/sup>. The first of these, termed the Halifax protocol, includes a 96-well tray platform, shortened wash times and incubations, and an increased serum to cell suspension volume ratio. The second, known as the Halifaster protocol, is proven to improve lymphocyte purity compared to density gradient centrifugation and to reduce the cell isolation time by approximately 40%. These methods are seeing increased uptake, along with various assays based on Luminex\u00ae technology, which have utility for both DSA testing and tissue typing.<\/p>\n<h4><strong>Anti-Human IgG F(ab\u2019)<sub>2<\/sub> antibodies from Jackson ImmunoResearch<\/strong><\/h4>\n<p>Jackson ImmunoResearch specializes in producing secondary antibodies for life science applications and offers Anti-Human IgG F(ab\u2019)<sub>2<\/sub> antibodies in two cross adsorptions. <a href=\"https:\/\/www.jacksonimmuno.com\/catalog\/products\/109-096-098\">109-096-098<\/a> is cross-adsorbed against Bovine, Horse and Mouse serum proteins, making it suitable for most FCXM protocols. <a href=\"https:\/\/www.jacksonimmuno.com\/catalog\/products\/109-096-170\">109-096-170<\/a> is cross-adsorbed against Bovine, Mouse, and Rabbit serum proteins, and is indicated for DSA testing in patients undergoing thymoglobulin immunosuppression (to prevent transplant rejection), for whom it is essential to prevent unwanted reactivity to circulating rabbit Immunoglobulin.<\/p>\n<h3>References<\/h3>\n<ol>\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK459467\/\">Cruz-Tapias P, Castiblanco J, Anaya JM. Major histocompatibility complex: Antigen processing and presentation. In: Anaya JM, Shoenfeld Y, Rojas-Villarraga A, et al., editors. Autoimmunity: From Bench to Bedside [Internet]. Bogota (Colombia): El Rosario University Press; 2013 Jul 18. Chapter 10.<span class=\"bk_cite_avail\">\u00a0Available from: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK459467\/<\/span><\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/17326240\/\">Choo S. Y. (2007). The HLA system: genetics, immunology, clinical testing, and clinical implications.\u00a0<i>Yonsei medical journal<\/i>,\u00a0<i>48<\/i>(1), 11\u201323. https:\/\/doi.org\/10.3349\/ymj.2007.48.1.11\/<\/a><\/li>\n<li><a href=\"https:\/\/www.ebi.ac.uk\/ipd\/imgt\/hla\/about\/statistics\/\">https:\/\/www.ebi.ac.uk\/ipd\/imgt\/hla\/about\/statistics\/<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31335997\/\">Mulley, W. R., Hudson, F., Lee, D., &amp; Holdsworth, R. F. (2019). Tissue typing for kidney transplantation for the general nephrologist.\u00a0<i>Nephrology (Carlton, Vic.)<\/i>,\u00a0<i>24<\/i>(10), 997\u20131000. https:\/\/doi.org\/10.1111\/nep.13637\/<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34479742\/\">Osoegawa, K., Montero-Mart\u00edn, G., Mallempati, K. C., Bauer, M., Milius, R. P., Maiers, M., Fern\u00e1ndez-Vi\u00f1a, M. A., &amp; Mack, S. J. (2021). Challenges for the standardized reporting of NGS HLA genotyping: Surveying gaps between clinical and research laboratories.\u00a0<i>Human immunology<\/i>,\u00a0<i>82<\/i>(11), 820\u2013828. https:\/\/doi.org\/10.1016\/j.humimm.2021.08.011\/<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28018342\/\">Tait B. D. (2016). Detection of HLA Antibodies in Organ Transplant Recipients &#8211; Triumphs and Challenges of the Solid Phase Bead Assay.\u00a0<i>Frontiers in immunology<\/i>,\u00a0<i>7<\/i>, 570. https:\/\/doi.org\/10.3389\/fimmu.2016.00570\/<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34237125\/\">Mahowald G. K. (2021). The CDC crossmatch in the era of flow cytometric cross-match and single antigen beads.\u00a0<i>Jornal brasileiro de nefrologia<\/i>,\u00a0<i>43<\/i>(3), 299\u2013300. https:\/\/doi.org\/10.1590\/2175-8239-JBN-2021-0110\/<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29109009\/\">Liwski, R. S., Greenshields, A. L., Conrad, D. M., Murphey, C., Bray, R. A., Neumann, J., &amp; Gebel, H. M. (2018). Rapid optimized flow cytometric crossmatch (FCXM) assays: The Halifax and Halifaster protocols.\u00a0<i>Human immunology<\/i>,\u00a0<i>79<\/i>(1), 28\u201338. https:\/\/doi.org\/10.1016\/j.humimm.2017.10.020\/<\/a><\/li>\n<\/ol>\n<p><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/company-news\/webinar-nanobodies-for-super-resolution-microscopy\/\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-10410 size-full\" style=\"width: 100%;\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Email-sig-Abberior-collab-3.jpg\" alt=\"\" width=\"600\" height=\"107\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Email-sig-Abberior-collab-3.jpg 600w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Email-sig-Abberior-collab-3-300x54.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/a><\/p>\n<hr \/>\n<p><span style=\"font-size: revert; color: initial;\">\u00a0<\/span><\/p>\n<p>\u00a0<\/p>\n<table class=\"table blogLinks\">\n<thead>\n<tr>\n<th class=\"span6\">Learn more:<\/th>\n<th class=\"span6\">Do more:<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td class=\"span6\"><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/directandindirectwesternblotting\/\">Indirect and direct Western blotting<\/a><\/td>\n<td class=\"span6\"><a href=\"https:\/\/jireurope.com\/home\/exhibitions\">Exhibition Schedule<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\"><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/chemiluminescent-western-blotting\/\">Chemiluminescence western blotting<\/a><\/td>\n<td class=\"span6\"><a href=\"https:\/\/jireurope.com\/home\/offers\/citeandwin\">Cite and Win<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\"><a class=\"row-title\" href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-admin\/post.php?post=3642&amp;action=edit\" aria-label=\"\u201cAn Introduction to Expansion Microscopy\u201d (Edit)\">An Introduction to Expansion Microscopy<\/a><\/td>\n<td class=\"span6\"><a href=\"https:\/\/jireurope.com\/home\/offers\/previous-winners\">Past Competition Winners<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n<p><\/p>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Download PDF Tissue typing, also known as human leukocyte antigen (HLA) testing, is the process of determining whether a donor tissue or organ will be compatible with its intended host. It involves comparing HLA expression between the prospective donor and the recipient to determine how similar the two antigen patterns are. The closer the match, [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":11,"featured_media":10425,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"content-type":""},"categories":[20],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/10420"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=10420"}],"version-history":[{"count":8,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/10420\/revisions"}],"predecessor-version":[{"id":10512,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/10420\/revisions\/10512"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/10425"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=10420"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/categories?post=10420"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/tags?post=10420"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}