{"id":10963,"date":"2024-07-04T04:42:40","date_gmt":"2024-07-04T08:42:40","guid":{"rendered":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/?p=10963"},"modified":"2025-10-29T06:56:09","modified_gmt":"2025-10-29T10:56:09","slug":"abberior-minflux","status":"publish","type":"post","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/abberior-minflux\/","title":{"rendered":"abberior Flux dyes and JIR AffiniPure-VHH\u2122 antibodies"},"content":{"rendered":"\n\n\n\t<div class=\"dkpdf-button-container\" style=\" text-align:right \">\n\n\t\t<a class=\"dkpdf-button\" href=\"\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/10963?pdf=10963\" target=\"_blank\"><span class=\"dkpdf-button-icon\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span> Download PDF<\/a>\n\n\t<\/div>\n\n\n\n\n\n<style>.entry p, .entry ol{font-size: 1rem;}.entry h3{color:#009fe3;margin-top:0}.entry h4{color:#009453;margin-top:1.5rem}.entry h5{color: #003a5c; font-size: 1.05rem;margin-top:1.5rem;}.entry figure{margin:32px auto;border:1px solid #ccc}.entry figure img{width:100%;display:block;margin:0 auto}.entry figcaption{font-size:.875rem;line-height:1.35rem;padding:10px;color:#222}.entry .blog-tbl{margin:1rem auto;caption-side:bottom}.entry .blog-tbl td,.entry .blog-tbl th{padding:10px 9px;border:1px solid #00172b;text-align:left}.entry .blog-tbl td{border-color:#003a5c}.entry .blog-tbl th{background-color:#00172b;color:#fff;border-left-color:#fff;border-right-color:#fff}.entry .blog-tbl th p{color:#fff}.entry .blog-tbl th:first-of-type{border-left-color:#00172b}.entry .blog-tbl th:last-of-type{border-right-color:#00172b}.entry .blog-tbl p{text-align:left;margin:0}.entry .box-note{border:2px solid #009453;padding:12px;margin:1rem 0}.entry .box-note p{margin:0;padding:0}.entry .styled-list{list-style-type:none}.entry .styled-list li{margin-top:1rem;line-height:22px;font-size:1rem;}.entry .styled-list li::before{font-family:\"Font Awesome 5 Pro\";display:inline-block;content:\"\\f3c5\";-webkit-transform:rotate(-90deg);transform:rotate(-90deg);margin-left:-20px;margin-right:11px;font-size:.75rem;color:#ed7004;font-weight:600}.entry .styled-list ol li::before{display:none}.entry .styled-list li>ul li::before{font-weight:200}.entry .overview{width:-webkit-fit-content;width:-moz-fit-content;width:fit-content;padding:16px;margin:1rem auto;border:1px solid #eee}.entry .overview hr{margin-top:14px}.entry .overview-text{text-align:center;font-size:1rem;margin:0}.entry .btn-sq{color:#fff;font-size:.9rem;font-weight:600;border:2px solid rgb(237, 112, 4);padding:7px 13px;background:rgb(237, 112, 4);cursor:pointer;text-align:center;line-height:1.5rem;margin:0 auto;}.entry .btn-sq:hover{text-decoration:none;color:rgb(237, 112, 4);background:#fff;}.entry .btn-container{display:flex;width:100%;margin:1.3rem 0;}.entry .btn-container .fa-solid, .entry .btn-container .fa-duotone{margin-right:10px;}@media(max-width: 768px){.entry .btn-sq{font-size:1.05rem;}}<\/style>\n<style>.interviewee{text-align:center}.interviewee img{display:block;border-radius:50%;margin:0 auto;width:200px;height:200px}.fig3-images{position:relative}.fig3-images:hover .fig-zoom{display:initial}.fig-fullscreen{position:fixed;background:rgba(30,30,30,.95);width:100vw;height:100vh;top:0;left:0;z-index:1000;display:flex}.fig-fullscreen-images{max-width:1438px;justify-content:space-between;margin:auto}.fig-fullscreen-images-inner{display:flex}.fig-fullscreen-images img:first-of-type,.fig-fullscreen-images img:nth-of-type(2){border-right:1px solid #ccc}.fig-fullscreen-text{background:#fff;margin-left:auto;margin-right:auto;padding:12px;border-top:1px solid #ccc}.fig-fullscreen-text p{margin:0}.body-hide{overflow-y:hidden}.fig-zoom,.fig-close{background:#ed7004;border:2px solid #ed7004;color:#fff;font-size:1.2rem;width:40px;height:40px}.fig-zoom:hover,.fig-close:hover{background-color:#fff;color:#ed7004}.fig-zoom{position:absolute;right:5px;top:5px;display:none}.fig-close{position:fixed;right:20px;top:20px}.entry .fig-add-space{max-width:550px}.entry .fig-add-space img{width:95%;display:block;margin-left:auto;margin-right:auto;margin-top:2.5%}.entry .fig4 img,.entry .fig5 img{display:inline-block;width:calc(50% - 1px)}.entry .fig4 img:last-of-type,.entry .fig5 img:last-of-type{border-left:2px solid #ccc}.entry h4{margin-top:2.5rem;line-height:24px}.fig3{max-width:380px}@media(min-width: 768px){.fig5 img:first-of-type{border-top:27px solid #090f08;border-bottom:27px solid #090f08}}@media(min-width: 590px){.entry .fig3{max-width:100%}.entry .fig3 .fig3-images{display:flex}.entry .fig3 .fig3-images img{flex-grow:1;width:1px}}@media(max-width: 1450px){.fig-fullscreen-images{width:100%}.fig-fullscreen-images img{flex-grow:1;width:1px}}@media(max-width: 767px){.entry .fig5{max-width:420px}.entry .fig5 img{display:block;width:100%}.entry .fig5 img:last-of-type{border-left:none;border-top:2px solid #ccc}}@media(max-width: 550px){.entry .fig4{max-width:420px}.entry .fig4 img{display:block;width:100%}.entry .fig4 img:last-of-type{border-left:none;border-top:2px solid #ccc}}.js-hide{display:none}<\/style>\n<p><script src=\"https:\/\/kit.fontawesome.com\/904923013f.js\" crossorigin=\"anonymous\"><\/script><\/p>\n<div class=\"entry\">\n<h2>Indirect immunofluorescence staining is a pivotal method for biomolecule labeling, yet its efficacy for super-resolution microscopy can be hindered by large complexes that form between primary and secondary antibodies and fluorophores. This leads to large linkage errors and artifacts due to the distance between the label and the target protein; therefore, solutions that increase the proximity between the fluorophore and the target molecule are crucial. We present a groundbreaking solution by combining abberior FLUX dyes with Jackson ImmunoResearch&#8217;s polyclonal AffiniPure-VHH\u2122, secondary antibodies. Specifically optimized for single-molecule localization techniques such as MINFLUX and STORM microscopy, abberior FLUX dyes ensure precise localization. Paired with JIR&#8217;s polyclonal VHH fragment antibodies (nanobodies), which bind to multiple sites of the primary antibody, they ensure remarkable signal amplification, resulting in captivating and informative images. This innovative combination heralds a new era in immunofluorescence labeling, offering superior performance, full flexibility, and enhanced visualization capabilities. Read our interview with abberior and Jackson ImmunoResearch on how these dyes and antibodies compliment each other&#8230;<\/h2>\n<h4>Interviewees<\/h4>\n<div class=\"interviewee\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-full wp-image-10967\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/df.jpg\" alt=\"\" width=\"292\" height=\"292\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/df.jpg 292w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/df-150x150.jpg 150w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/df-45x45.jpg 45w\" sizes=\"(max-width: 292px) 100vw, 292px\" \/><\/p>\n<h5>Dave Fancy, Ph.D Jackson ImmunoResearch<\/h5>\n<p>Dave is the President of Jackson ImmunoResearch Laboratories. After gaining his Ph.D. from the University of Texas, Dave has specialized in the development of novel antibody conjugates of dyes, metal chelates, enzymes and DNA for use in the research. He has extensive experience in developing immunological products and services, with a focus on commercialization for the biotech research, diagnostic communities.<\/p>\n<\/div>\n<hr \/>\n<div class=\"interviewee\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-full wp-image-10968\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/eg.jpg\" alt=\"\" width=\"300\" height=\"300\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/eg.jpg 300w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/eg-150x150.jpg 150w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/eg-45x45.jpg 45w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<h5>Evelyn Garlick, Ph.D.\u00a0abberior Instruments<\/h5>\n<p>Evelyn comes from the UK and completed her Ph.D. at the Universities of Birmingham and Nottingham in 2022. Since then, she has been a part of the MINFLUX applications team at abberior Instruments, working to help users get the most out of their MINFLUX microscopes.<\/p>\n<\/div>\n<hr \/>\n<div class=\"interviewee\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-full wp-image-10969\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fg.jpg\" alt=\"\" width=\"300\" height=\"300\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fg.jpg 300w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fg-150x150.jpg 150w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fg-45x45.jpg 45w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<h5>Florian Grimm, Ph.D. abberior dyes &amp; labels<\/h5>\n<p>Florian, with a background in biochemistry and chemistry, was abberior&#8217;s first employee. With over a decade of experience with abberior dyes, labels, and microscopes, he now serves as a Business Developer. Florian is dedicated to finding innovative probes and maintaining key business relationships, driving abberior&#8217;s mission forward.<\/p>\n<\/div>\n<h4>How does the format of AffiniPure VHH\u2122 secondary antibodies help?<\/h4>\n<p>Fancy: The novel format of the VHH fragment is a 10<sup>th<\/sup> of the size of a conventional Ig antibody, this is good news if you want precise labeling where the fluorophore is close to your target protein because the primary\/secondary complex is considerably reduced in size.<\/p>\n<figure class=\"fig-add-space\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-10970\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fig-1-1.jpg\" alt=\"\" width=\"700\" height=\"369\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fig-1-1.jpg 1124w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fig-1-1-300x158.jpg 300w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fig-1-1-1024x539.jpg 1024w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><figcaption><strong>Figure 1: <\/strong>VHH fragment antibodies in comparison to conventional whole lg antibodies<\/figcaption><\/figure>\n<h4>I\u2019ve used monoclonal nanobodies, why should I use a polyclonal one?<\/h4>\n<p>Fancy: The limitations of monoclonal antibodies are apparent when you need to achieve maximum brightness. The signal amplification achieved with polyclonal AffiniPure- VHH\u2122 antibodies comes from the heterogenous population of antibodies, with different paratopes able to bind at many sites across the primary antibody ensuring it is maximally decorated with signal producing fluors. Figure 2 demonstrates the advantages of polyclonal VHH antibodies, binding across the whole of the primary antibody, rather than being limited to a specific site\/region\/sequence, enabling higher labeling efficiency and brighter signal than the monoclonal format.<\/p>\n<figure class=\"fig-add-space\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-10971\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fig2.jpg\" alt=\"\" width=\"500\" height=\"265\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fig2.jpg 748w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fig2-300x159.jpg 300w\" sizes=\"(max-width: 500px) 100vw, 500px\" \/><figcaption><strong>Figure 2: <\/strong>The polyclonal format of AffiniPure-VHH\u2122 ensures maximum signal generated without compromising the signal density\/resolution.<\/figcaption><\/figure>\n<h4>I\u2019ve got multiple targets; will these work together?<\/h4>\n<p>Fancy: JIR AffiniPure-VHH\u2122 antibodies are cross-adsorbed against commonly used species to enhance specificity and thus reduce off-target labeling, they can be used in combination to generate exquisitely specific multiple labeling images.<\/p>\n<h4>What other benefits does the indirect method offer?<\/h4>\n<p>Fancy: Indirect labeling using conjugated AffiniPure-VHH\u2122 Secondaries gives researchers access to a greater range of fluorophores, with AffiniPure VHH\u2122 Secondaries available conjugated to fluors from ultraviolet to far-red researchers can pick the right characteristics for their experiments which is useful for those identifying multiple target proteins or seeking dyes suitable for SRM and SM techniques.<\/p>\n<div class=\"btn-container\"><a class=\"btn-sq\" href=\"\/technical\/products\/AffiniPure-VHH-secondary-antibodies\"><i class=\"fa-solid fa-graduation-cap\"><\/i>Learn more about AffinPure-VHH\u2122 Secondary Antibodies<\/a><\/div>\n<h4>What is MINFLUX microscopy?<\/h4>\n<p>Garlick: MINFLUX (MINimal photon FLUXes) is a single molecule localization technique that finds fluorophores using an excitation minima rather than maxima. This difference is what makes MINFLUX the most precise and photon-efficient way of localizing fluorescent molecules, capable of achieving 1 &#8211; 3 nm localization precision in all three dimensions. The unparalleled precision afforded by MINFLUX nanoscopy has many applications, giving impressive insights into biological structures at the nanoscale.<\/p>\n<h4>What are FLUX dyes?<\/h4>\n<p>Grimm: FLUX dyes are organic fluorescent molecules that have been developed and tested for MINFLUX microscopy. The main feature of this particular class of dyes is that they start to blink through the use of a special buffer system. This blinking feature enables our dyes to oscillate between an ON and OFF state, a crucial aspect as it ensures that not all dyes are active simultaneously, allowing for precise localization of individual dye molecules over time.<\/p>\n<p>But the versatility of abberior FLUX dyes doesn&#8217;t end there. They&#8217;re also compatible with other single-molecule localization microscopy techniques such as STORM, opening doors to many research possibilities.<\/p>\n<div class=\"fig-fullscreen js-hide\">\n<div class=\"fig-fullscreen-images\">\n<div class=\"fig-fullscreen-images-inner\"><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fig3a.jpg\" alt=\"\" \/><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fig3b.jpg\" alt=\"\" \/><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fig3c.jpg\" alt=\"\" \/><\/div>\n<div class=\"fig-fullscreen-text\">\n<p><strong>Figure 3: <\/strong>Absorption and fluorescence spectra of abberior FLUX dyes.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<figure class=\"fig3\">\n<div class=\"fig3-images\"><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fig3a.jpg\" alt=\"\" \/><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fig3b.jpg\" alt=\"\" \/><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/fig3c.jpg\" alt=\"\" \/><button class=\"fig-zoom\"><i class=\"fa-solid fa-arrow-up-right-and-arrow-down-left-from-center\"><\/i><\/button><\/div><figcaption><strong>Figure 3: <\/strong>Absorption and fluorescence spectra of abberior FLUX dyes.<\/figcaption><\/figure>\n<h4>What is the advantage of FLUX dyes conjugated to AffiniPure-VHH\u2122 antibodies?<\/h4>\n<p>Garlick: Dave explained that when using traditional primary and secondary antibodies, the distance from your epitope of interest to the fluorophore on your secondary can be ~ 20 nm. This is called linkage error. If you have a technique like MINFLUX that is capable of localizing a fluorophore with a precision of &lt; 3 nm, reducing this linkage error is essential to better understand the arrangement of the epitope. AffiniPure-VHH\u2122 Secondary antibodies can improve the linkage error, being much smaller than conventional Ig, they bring the fluorophore closer to the epitope, whilst keeping the flexibility of a traditional immunostaining approach.<\/p>\n<h4>How do i use abberior FLUX dyes conjugated to JIR AffiniPure-VHH Fragment antibodies?<\/h4>\n<p>Garlick: FLUX-conjugated VHH fragment secondary antibodies can easily be incorporated into existing staining routines in place of your conventional secondary antibody. \u00a0A detailed protocol can be found here: <a href=\"https:\/\/abberior.rocks\/expertise\/protocols\/nanobody-protocol\/\">https:\/\/abberior.rocks\/expertise\/protocols\/nanobody-protocol\/<\/a>.<\/p>\n<h4>What is important when preparing a MINFLUX sample?<\/h4>\n<p>Garlick: Some special attention to your sample can help you achieve great quality MINFLUX results. In general, steps should be taken during labeling to limit background from both autofluorescence and unspecific labeling or binding. Make sure you incorporate a robust blocking and washing routine.<\/p>\n<h4>What dye to choose for single-color and which pair for two-color MINFLUX imaging?<\/h4>\n<p>Garlick: Our go-to dye recommendation for single-color imaging is FLUX 647. The best pair for two-color MINFLUX is FLUX 640 and FLUX 680 &#8211; these fluorophores are nicely spectrally separated in their emission and perform well in the same blinking buffer conditions, allowing simultaneous imaging of the two labels with the same excitation line.<\/p>\n<figure class=\"fig4\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-10986 size-full\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/FLUX-647-ELYS-Nb-confocal-ROI.jpg\" alt=\"\" width=\"225\" height=\"230\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/FLUX-647-ELYS-Nb-confocal-ROI.jpg 225w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/FLUX-647-ELYS-Nb-confocal-ROI-45x45.jpg 45w\" sizes=\"(max-width: 225px) 100vw, 225px\" \/><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-medium wp-image-10987\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/FLUX-647-ELYS-Nb-MINFLUX-293x300.jpg\" alt=\"\" width=\"293\" height=\"300\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/FLUX-647-ELYS-Nb-MINFLUX-293x300.jpg 293w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/FLUX-647-ELYS-Nb-MINFLUX-45x45.jpg 45w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/FLUX-647-ELYS-Nb-MINFLUX.jpg 450w\" sizes=\"(max-width: 293px) 100vw, 293px\" \/><figcaption><strong>Figure 4: <\/strong>2D MINFLUX nanoscopy of the nuclear pore complex subunits, labeled with abberior FLUX 647 conjugated to JIR AffiniPure-VHH Fragment antibodies. \u00a0In contrast to confocal microscopy, 2D MINFLUX allows visualization of the shape and arrangement of individual nuclear pore complex subunits. Here, we reach localization precisions of ~2 nm in raw localization data.<\/figcaption><\/figure>\n<figure class=\"fig5\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-medium wp-image-10988\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Nb-FLUX-640-FLUX-680-mito-confocal-ROI-300x159.jpg\" alt=\"\" width=\"300\" height=\"159\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Nb-FLUX-640-FLUX-680-mito-confocal-ROI-300x159.jpg 300w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Nb-FLUX-640-FLUX-680-mito-confocal-ROI.jpg 408w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-medium wp-image-10989\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Nb-FLUX-640-FLUX-680-mito-MINFLUX-2-color-300x206.jpg\" alt=\"\" width=\"300\" height=\"206\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Nb-FLUX-640-FLUX-680-mito-MINFLUX-2-color-300x206.jpg 300w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Nb-FLUX-640-FLUX-680-mito-MINFLUX-2-color.jpg 450w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><figcaption><strong>Figure 5: <\/strong>Two-color 3D MINFLUX revealing an inner and outer mitochondrial membrane marker. Cultured mammalian cells labeled with indirect immunofluorescence using JIR AffiniPure-VHH Fragment antibodies coupled to abberior FLUX 640 (orange) and FLUX 680 (cyan). MINFLUX enables the visualization and separation of both structures.<\/figcaption><\/figure>\n<a style=\"display:block;margin:1rem auto 10px;\" href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/AffiniPure-VHH-secondary-antibodies\"><img class=\"aligncenter wp-image-9850 size-full\" style=\"width: 100%;\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/VHH-affinipure-Q4-push_creatives_GIF_email_600x107_01.gif\" alt=\"\"><\/a>\n<table class=\"table blogLinks\">\n<thead>\n<tr>\n<th class=\"span6\">Learn more:<\/th>\n<th class=\"span6\">Do more:<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td class=\"span6\"><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/directandindirectwesternblotting\/\">Indirect and direct Western blotting<\/a><\/td>\n<td class=\"span6\"><a href=\"https:\/\/jacksonimmuno.com\/home\/exhibitions\">Exhibition Schedule<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\"><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/chemiluminescent-western-blotting\/\">Chemiluminescence western blotting<\/a><\/td>\n<td class=\"span6\"><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/western-blotting-guide\/\">Western blotting guide<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\"><a class=\"row-title\" href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/expansionmicroscopy\/\">An Introduction to Expansion Microscopy<\/a><\/td>\n<td class=\"span6\"><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/elisa-guide\/\">ELISA guide<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><script>const zoomBtn=document.querySelectorAll(\".fig-zoom\"),fullscreenImageContainer=document.querySelectorAll(\".fig-fullscreen\"),zoomClose=document.querySelectorAll(\".fig-close\"),body=document.getElementsByTagName(\"BODY\");zoomBtn.forEach((e,o)=>{e.addEventListener(\"click\",()=>{fullscreenImageContainer[o].classList.remove(\"js-hide\"),body[0].classList.add(\"body-hide\")})}),zoomClose.forEach((e,o)=>{e.addEventListener(\"click\",()=>{fullscreenImageContainer[o].classList.add(\"js-hide\"),body[0].classList.remove(\"body-hide\")})});<\/script><\/p>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Download PDF Indirect immunofluorescence staining is a pivotal method for biomolecule labeling, yet its efficacy for super-resolution microscopy can be hindered by large complexes that form between primary and secondary antibodies and fluorophores. This leads to large linkage errors and artifacts due to the distance between the label and the target protein; therefore, solutions that [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":3,"featured_media":10950,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"content-type":""},"categories":[3,16],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/10963"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=10963"}],"version-history":[{"count":30,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/10963\/revisions"}],"predecessor-version":[{"id":11484,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/10963\/revisions\/11484"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/10950"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=10963"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/categories?post=10963"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/tags?post=10963"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}