{"id":11222,"date":"2025-03-19T07:33:24","date_gmt":"2025-03-19T11:33:24","guid":{"rendered":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/?p=11222"},"modified":"2025-05-27T10:24:10","modified_gmt":"2025-05-27T14:24:10","slug":"imaging-with-jir-antibodies","status":"publish","type":"post","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/imaging-with-jir-antibodies\/","title":{"rendered":"Imaging with Jackson ImmunoResearch Secondary Antibodies"},"content":{"rendered":"\n\n\n\t<div class=\"dkpdf-button-container\" style=\" text-align:right \">\n\n\t\t<a class=\"dkpdf-button\" href=\"\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/11222?pdf=11222\" target=\"_blank\"><span class=\"dkpdf-button-icon\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span> Download PDF<\/a>\n\n\t<\/div>\n\n\n\n\n\n<style>.entry p, .entry ol{font-size: 1rem;}.entry h3{color:#009fe3;margin-top:0}.entry h4{color:#009453;margin-top:1.5rem}.entry h5{color: #003a5c; font-size: 1.05rem;margin-top:1.5rem;}.entry figure{margin:32px auto;border:1px solid #ccc}.entry figure img{width:100%;display:block;margin:0 auto}.entry figcaption{font-size:.875rem;line-height:1.35rem;padding:10px;color:#222}.entry .blog-tbl{margin:1rem auto;caption-side:bottom}.entry .blog-tbl td,.entry .blog-tbl th{padding:10px 9px;border:1px solid #00172b;text-align:left}.entry .blog-tbl td{border-color:#003a5c}.entry .blog-tbl th{background-color:#00172b;color:#fff;border-left-color:#fff;border-right-color:#fff}.entry .blog-tbl th p{color:#fff}.entry .blog-tbl th:first-of-type{border-left-color:#00172b}.entry .blog-tbl th:last-of-type{border-right-color:#00172b}.entry .blog-tbl p{text-align:left;margin:0}.entry .box-note{border:2px solid #009453;padding:12px;margin:1rem 0}.entry .box-note p{margin:0;padding:0}.entry .styled-list{list-style-type:none}.entry .styled-list li{margin-top:1rem;line-height:22px;font-size:1rem;}.entry .styled-list li::before{font-family:\"Font Awesome 5 Pro\";display:inline-block;content:\"\\f3c5\";-webkit-transform:rotate(-90deg);transform:rotate(-90deg);margin-left:-20px;margin-right:11px;font-size:.75rem;color:#ed7004;font-weight:600}.entry .styled-list ol li::before{display:none}.entry .styled-list li>ul li::before{font-weight:200}.entry .overview{width:-webkit-fit-content;width:-moz-fit-content;width:fit-content;padding:16px;margin:1rem auto;border:1px solid #eee}.entry .overview hr{margin-top:14px}.entry .overview-text{text-align:center;font-size:1rem;margin:0}.entry .btn-sq{color:#fff;font-size:.9rem;font-weight:600;border:2px solid rgb(237, 112, 4);padding:7px 13px;background:rgb(237, 112, 4);cursor:pointer;text-align:center;line-height:1.5rem;margin:0 auto;}.entry .btn-sq:hover{text-decoration:none;color:rgb(237, 112, 4);background:#fff;}.entry .btn-container .fa-solid, .entry .btn-container .fa-duotone{margin-right:10px;}@media(max-width: 768px){.entry .btn-sq{font-size:1.05rem;}}<\/style>\n<style>.entry h4{margin-top: 2rem;}.entry ol li{line-height: 1.6rem;margin-top:1rem;}<\/style>\n<p><script src=\"https:\/\/kit.fontawesome.com\/904923013f.js\" crossorigin=\"anonymous\"><\/script><\/p>\n<div class=\"entry\">\n<h2>Imaging using immunostaining is a commonly used technique that can elucidate many details about how a process functions in situ. It is applied in many fields, including cancer and neuroscience research, to create a better understanding of biological processes. Although immunostaining is a robust and straightforward proposition, as the complexity of the experiment increases to dissect more complicated questions, experimental design requires reagents that enable specific detection. The following article introduces how Jackson ImmunoResearch cross-adsorbed antibodies and NEW Anti-GFP antibodies, can benefit your research.<\/h2>\n<h4>Cross-adsorbed antibodies for exquisite differentiation<\/h4>\n<p>Secondary antibodies raised against one species may recognize epitopes on other species\u2019 immunoglobulins through structural similarities, known as homology. This cross-reactivity can be problematic and cause background tissue staining or signal by recognizing a non-target primary antibody. When experiments combine material from multiple species, such as presented by sample species or multiple primary antibodies presenting different hosts, secondary antibodies with minimal cross-reactivity (min X) to serum proteins from non-target species are necessary to avert background from cross-reactivity.<\/p>\n<figure style=\"max-width:400px;\">\n        <img decoding=\"async\" loading=\"lazy\" width=\"400\" height=\"453\" class=\"aligncenter wp-image-2109 size-full\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/NRC2_tub_2des400-1.jpg\" alt=\"\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/NRC2_tub_2des400-1.jpg 400w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/NRC2_tub_2des400-1-265x300.jpg 265w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/><figcaption><strong>Figure 1:<\/strong> Primary culture of cardiac cells.<br \/>\n<em style=\"font-size: revert; color: initial;\">Determining Cardiomyocytes from heart primary cultures: 3 cell types are identified in this image. Red only cells show fibroblasts (positive for Rat Anti-tubulin); Smooth muscle cells are positive for both tubulin (red) and desmin (blue). Cardiomyocytes are identified in this image by their exhibition of green stripes, positive for MyBP-C. <\/em><br \/>\n<span style=\"font-size: revert; color: initial;\">To find out more about Dr Ehler\u2019s research read <\/span><a style=\"font-size: revert;\" href=\"https:\/\/www.jacksonimmuno.com\/company-news\/distinguish-cell%E2%80%A6primary-cultures\/\">here<\/a>.<br \/>\n<span class=\"imageCredit\">Dr Elisabeth Ehler, King&#8217;s College London<\/span><\/figcaption><\/figure>\n<p>Cross-absorbed antibodies from Jackson ImmunoResearch are designated as &#8220;(min X &#8230; Sr Prot)&#8221; in their product description. These antibodies exhibit minimal cross-reactivity with the species detailed in the parenthesis, whether presented by the experimental antibodies or by endogenous proteins in the sample. For example, AffiniPure\u00ae Donkey Anti-Mouse IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Rat, Shp Sr Prot) antibody has been cross-adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, rat and sheep IgG and serum proteins and is suitable for experiments where those species are used. You can learn more about multiple labeling and cross-adsorbed antibodies by following the links below.<\/p>\n<div class=\"btn-container vertical-btns\">\n        <a style=\"max-width: 500px;\" href=\"\/technical\/products\/cross-adsorbed-secondary-antibodies\" class=\"btn-sq\"><i class=\"fa-solid fa-graduation-cap\"><\/i>Cross-adsorbed (min X) secondary antibodies and cross-reactivity<\/a><br \/>\n<a style=\"max-width: 500px;\" href=\"\/technical\/products\/protocols\/multiple-labeling\" class=\"btn-sq\"><i class=\"fa-solid fa-graduation-cap\"><\/i>Multiple labeling<\/a><\/div>\n<h4>Anti-GFP Antibodies for the detection of GFP proteins<\/h4>\n<p>Jackson ImmunoResearch AffiniPure\u2122 Rabbit Anti-GFP is an affinity-purified polyclonal antibody raised in rabbit and is specific for <i>Aequorea victoria<\/i> Green Fluorescent Protein (<i>av<\/i>GFP) and its derivatives, including EGFP, ECFP, and EYFP. These antibodies are versatile reagents and may be used in a wide range of applications, including ELISA, IHC, ICC, IP, Flow cytometry, and WB.<\/p>\n<h5>Imaging with Anti-GFP<\/h5>\n<h6 style=\"font-size:14px;\">Conjugates for immunofluorescence<\/h6>\n<p>This Anti-GFP antibody is available conjugated to a range of reporter molecules, including Alexa Fluor\u00ae dyes to enable indirect detection for bright target signal and spectacular signal amplification.<\/p>\n<p>They enable circumvention of autofluorescence or equipment limitations, such as when a laser line or channel is occupied by another target, making them useful when detecting multiple targets in the same experiment.<\/p>\n<div class=\"btn-container\">\n        <a href=\"\/technical\/products\/groups\/anti-gfp\" class=\"btn-sq\"><i class=\"fa-duotone fa-book-open-reader\"><\/i>Learn more about Anti-GFP antibodies<\/a><\/div>\n<figure style=\"max-width:450px;\">\n        <img decoding=\"async\" loading=\"lazy\" width=\"700\" height=\"466\" class=\"aligncenter wp-image-11033 size-full\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Picture3.jpg\" alt=\"\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Picture3.jpg 700w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Picture3-300x200.jpg 300w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><figcaption><strong>Figure 2:<\/strong> Immunofluorescence microscopy of Hela cells expressing a transiently transfected GFP fusion protein. Alexa Fluor\u00ae 647 Rabbit Anti-GFP detection of EGFP tagged Tubulin protein.<\/figcaption><\/figure>\n<p><a href=\"\/technical\/products\/groups\/anti-gfp\"><img decoding=\"async\" loading=\"lazy\" width=\"729\" height=\"91\" class=\"aligncenter wp-image-11094 size-full\" style=\"width: 100%;\" src=\"\/secondary-antibody-resource\/wp-content\/uploads\/24-Q2-GFP-Ads-728x90-B.jpg\" alt=\"\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/24-Q2-GFP-Ads-728x90-B.jpg 729w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/24-Q2-GFP-Ads-728x90-B-300x37.jpg 300w\" sizes=\"(max-width: 729px) 100vw, 729px\" \/><\/a><\/p>\n<\/div>\n<table class=\"table blogLinks\">\n<thead>\n<tr>\n<th class=\"span6\">Learn more:<\/th>\n<th class=\"span6\">Do more:<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td class=\"span6\"><a href=\"\/secondary-antibody-resource\/immuno-techniques\/directandindirectwesternblotting\/\">Indirect and direct Western blotting<\/a><\/td>\n<td class=\"span6\"><a href=\"https:\/\/jacksonimmuno.com\/home\/exhibitions\">Exhibition Schedule<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\"><a href=\"\/secondary-antibody-resource\/immuno-techniques\/chemiluminescent-western-blotting\/\">Chemiluminescence western blotting<\/a><\/td>\n<td class=\"span6\"><a href=\"\/secondary-antibody-resource\/immuno-techniques\/western-blotting-guide\/\">Western blotting guide<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\"><a class=\"row-title\" href=\"\/secondary-antibody-resource\/technical-tips\/expansionmicroscopy\/\">An Introduction to Expansion Microscopy<\/a><\/td>\n<td class=\"span6\"><a href=\"\/secondary-antibody-resource\/immuno-techniques\/elisa-guide\/\">ELISA guide<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Download PDF Imaging using immunostaining is a commonly used technique that can elucidate many details about how a process functions in situ. It is applied in many fields, including cancer and neuroscience research, to create a better understanding of biological processes. Although immunostaining is a robust and straightforward proposition, as the complexity of the experiment [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":3,"featured_media":11141,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"content-type":""},"categories":[3,16],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/11222"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=11222"}],"version-history":[{"count":17,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/11222\/revisions"}],"predecessor-version":[{"id":11241,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/11222\/revisions\/11241"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/11141"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=11222"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/categories?post=11222"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/tags?post=11222"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}