{"id":11456,"date":"2025-10-10T09:37:00","date_gmt":"2025-10-10T13:37:00","guid":{"rendered":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/?p=11456"},"modified":"2025-12-31T22:21:47","modified_gmt":"2026-01-01T03:21:47","slug":"25-off-rabbit-anti-gfp-antibodies","status":"publish","type":"post","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/company-news\/25-off-rabbit-anti-gfp-antibodies\/","title":{"rendered":"Get 25% off AffiniPure\u00aeRabbit Anti-GFP Antibodies"},"content":{"rendered":"\n\n\n\t<div class=\"dkpdf-button-container\" style=\" text-align:right \">\n\n\t\t<a class=\"dkpdf-button\" href=\"\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/11456?pdf=11456\" target=\"_blank\"><span class=\"dkpdf-button-icon\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span> Download PDF<\/a>\n\n\t<\/div>\n\n\n\n\n\n<style>.entry p, .entry ol{font-size: 1rem;}.entry h3{color:#009fe3;margin-top:0}.entry h4{color:#009453;margin-top:1.5rem}.entry h5{color: #003a5c; font-size: 1.05rem;margin-top:1.5rem;}.entry figure{margin:32px auto;border:1px solid #ccc}.entry figure img{width:100%;display:block;margin:0 auto}.entry figcaption{font-size:.875rem;line-height:1.35rem;padding:10px;color:#222}.entry .blog-tbl{margin:1rem auto;caption-side:bottom}.entry .blog-tbl td,.entry .blog-tbl th{padding:10px 9px;border:1px solid #00172b;text-align:left}.entry .blog-tbl td{border-color:#003a5c}.entry .blog-tbl th{background-color:#00172b;color:#fff;border-left-color:#fff;border-right-color:#fff}.entry .blog-tbl th p{color:#fff}.entry .blog-tbl th:first-of-type{border-left-color:#00172b}.entry .blog-tbl th:last-of-type{border-right-color:#00172b}.entry .blog-tbl p{text-align:left;margin:0}.entry .box-note{border:2px solid #009453;padding:12px;margin:1rem 0}.entry .box-note p{margin:0;padding:0}.entry .styled-list{list-style-type:none}.entry .styled-list li{margin-top:1rem;line-height:22px;font-size:1rem;}.entry .styled-list li::before{font-family:\"Font Awesome 5 Pro\";display:inline-block;content:\"\\f3c5\";-webkit-transform:rotate(-90deg);transform:rotate(-90deg);margin-left:-20px;margin-right:11px;font-size:.75rem;color:#ed7004;font-weight:600}.entry .styled-list ol li::before{display:none}.entry .styled-list li>ul li::before{font-weight:200}.entry .overview{width:-webkit-fit-content;width:-moz-fit-content;width:fit-content;padding:16px;margin:1rem auto;border:1px solid #eee}.entry .overview hr{margin-top:14px}.entry .overview-text{text-align:center;font-size:1rem;margin:0}.entry .btn-sq{color:#fff;font-size:.9rem;font-weight:600;border:2px solid rgb(237, 112, 4);padding:7px 13px;background:rgb(237, 112, 4);cursor:pointer;text-align:center;line-height:1.5rem;margin:0 auto;}.entry .btn-sq:hover{text-decoration:none;color:rgb(237, 112, 4);background:#fff;}.entry .btn-container{display:flex;width:100%;margin:1.3rem 0;}.entry .btn-container .fa-solid, .entry .btn-container .fa-duotone{margin-right:10px;}@media(max-width: 768px){.entry .btn-sq{font-size:1.05rem;}}<\/style>\n<style>.entry h4{margin-top: 2rem;}.entry ol li{line-height: 1.6rem;margin-top:1rem;}<\/style>\n<style>.entry .full-alexa-tbl a{color:#fff;text-decoration:underline}.entry .full-alexa-tbl a:hover{color:#eee}.entry .caption{margin-top:4px;font-size:.9rem}.full-alexa-tbl{max-width:500px;margin-left:auto;margin-right:auto}.full-alexa-tbl tr{border-bottom:2px solid #fff}.full-alexa-tbl tbody tr td{background-color:#d4edfc}.full-alexa-tbl tbody tr td:last-child{border-left:2px solid #fff}.full-alexa-tbl tbody .fluo-green{background-color:#28e100}.full-alexa-tbl tbody .fluo-orange{background-color:#e17c00}.full-alexa-tbl tbody .fluo-red{background-color:#e10000}.full-alexa-tbl tbody .fluo-dark-red{background-color:#6c0000}.full-alexa-tbl caption{margin-top:4px;font-size:.9rem}#fig3{max-width:500px}<\/style>\n<p><script src=\"https:\/\/kit.fontawesome.com\/904923013f.js\" crossorigin=\"anonymous\"><\/script><\/p>\n<h2>Green Fluorescent Protein (GFP) is a protein tag commonly used in fusion proteins as a reporter molecule. GFP has utility across numerous applications, including quantification of gene expression, protein localization within living organisms, characterization of protein interactions, and as a biosensor. The addition of an Anti-GFP antibody can often improve experimental performance and overcome limitations that use of the tag itself may incur. Jackson ImmunoResearch AffiniPure\u00ae Rabbit Anti-GFP is an affinity-purified polyclonal antibody raised in rabbit. It can be used to detect <i>Aequorea victoria<\/i>&nbsp;Green Fluorescent Protein (<i>av<\/i>GFP) and its derivatives, including EGFP, ECFP, and EYFP. This Fall, we are offering 25% off all AffiniPure\u00ae Rabbit Anti-GFP antibodies and conjugates. Learn More.<\/h2>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-11487 size-full\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Homepage-Slider-25-Q4-GFP-Fall-Promotion-1.gif\" alt=\"\" width=\"706\" height=\"404\"><\/p>\n<h4>Versatile detection of the GFP protein across a range of applications<\/h4>\n<p>AffiniPure\u00ae Rabbit Anti-GFP antibody can be used to amplify the signal from GFP expressed by cells in Immunohistochemistry (IHC) or Immunofluorescence (IF) experiments. Our rabbit polyclonal Anti-GFP antibody may also be used for immunoprecipitation (IP), ELISA, ICC, Flow cytometry, and Western Blotting.<\/p>\n<p>For 25% off AffiniPure\u00aeRabbit Anti-GFP Antibodies between now and the 31st of December 2025*.<\/p>\n<p>Click the button below and fill in the form to receive your discount code.<\/p>\n<div class=\"btn-container\"><a class=\"btn-sq\" href=\"https:\/\/ch7ef.share.hsforms.com\/2Hd7ehpkNTAedIKpU1-kwGQ\"><i class=\"fa-solid fa-tags\"><\/i>Get 25% off AffiniPure\u00ae Rabbit Anti-GFP Antibodies<\/a><\/div>\n<p>Once you&#8217;ve filled in the form you will receive a discount code that can be used to redeem a 25% discount off AffiniPure\u00ae Rabbit Anti-GFP Antibodies until 31st December 2025. Terms and conditions apply, see below.<\/p>\n<h4 id=\"imaging\">Imaging with AffiniPure\u00ae Rabbit Anti-GFP Antibodies<\/h4>\n<h5 id=\"icc\">Conjugates for immunofluorescence<\/h5>\n<p>Jackson ImmunoResearch AffiniPure\u00ae Rabbit Anti-GFP antibody is available conjugated to a range of reporter molecules. For fluorescence imaging, Jackson ImmunoResearch Rabbit Anti-GFP antibodies conjugated to Alexa Fluor\u00ae dyes can deliver bright target signal and spectacular signal amplification.<\/p>\n<p>Anti-GFP antibody conjugates can add versatility to an assay when working around autofluorescence or equipment limitations, such as when another target occupies a laser line or channel. Using a fluorophore that emits in a different channel, Anti-GFP antibodies can circumvent these limitations, making them useful for detecting multiple targets in the same experiment.<\/p>\n<figure id=\"fig3\"><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/img\/static-pages\/2560x1702_rabbit-anti-gfp-fig2.jpg\" alt=\"\"><figcaption><strong>Figure 1:<\/strong>&nbsp;Immunofluorescence microscopy of HeLa cells expressing a transiently transfected GFP fusion protein. Alexa Fluor\u00ae 647 Rabbit Anti-GFP detection of EGFP-tagged Tubulin protein.<\/figcaption><\/figure>\n<table id=\"conjugates\" class=\"alexa-tbl full-alexa-tbl tbl-blue\">\n<caption>Table 1: Rabbit Anti-GFP conjugates available.<\/caption>\n<thead>\n<tr>\n<th>Conjugate<\/th>\n<th>Excitation Peak (nm)<\/th>\n<th>Emission Peak (nm)<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<th class=\"fluo-green\" scope=\"row\"><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/conjugate-selection\/alexa-fluor\/488\">Alexa Fluor\u00ae 488<\/a><\/th>\n<td>493<\/td>\n<td>519<\/td>\n<\/tr>\n<tr>\n<th class=\"fluo-orange multiple-fluors\" scope=\"row\"><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/conjugate-selection\/alexa-fluor\/555-568#af555\">Alexa Fluor\u00ae 555<\/a><\/th>\n<td>552<\/td>\n<td>572<\/td>\n<\/tr>\n<tr>\n<th class=\"fluo-orange\" scope=\"row\"><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/conjugate-selection\/r-pe\">R-Phycoerythrin, R-PE<\/a><\/th>\n<td>many, 488<\/td>\n<td>580<\/td>\n<\/tr>\n<tr>\n<th class=\"fluo-orange\" scope=\"row\"><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/conjugate-selection\/alexa-fluor\/555-568#af568\">Alexa Fluor\u00ae 568<\/a><\/th>\n<td>577<\/td>\n<td>602<\/td>\n<\/tr>\n<tr>\n<th class=\"fluo-orange\" scope=\"row\"><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/conjugate-selection\/alexa-fluor\/594\">Alexa Fluor\u00ae 594<\/a><\/th>\n<td>591<\/td>\n<td>614<\/td>\n<\/tr>\n<tr>\n<th class=\"fluo-red\" scope=\"row\"><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/conjugate-selection\/alexa-fluor\/647\">Alexa Fluor\u00ae 647<\/a><\/th>\n<td>651<\/td>\n<td>667<\/td>\n<\/tr>\n<tr>\n<th class=\"fluo-red\" scope=\"row\"><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/conjugate-selection\/alexa-fluor\/680\">Alexa Fluor\u00ae 680<\/a><\/th>\n<td>684<\/td>\n<td>702<\/td>\n<\/tr>\n<tr>\n<th class=\"fluo-dark-red\" scope=\"row\"><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/conjugate-selection\/alexa-fluor\/790\">Alexa Fluor\u00ae 790<\/a><\/th>\n<td>792<\/td>\n<td>803<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<div class=\"btn-container\"><a class=\"btn-sq\" href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/groups\/anti-gfp\/rabbit\"><i class=\"fa-solid fa-graduation-cap\"><\/i>Learn more about AffiniPure\u00aeRabbit Anti-GFP Antibodies<\/a><\/div>\n<h4>Terms and conditions<\/h4>\n<ul class=\"styled-list\">\n<li>This offer is only available for direct purchases with Jackson ImmunoResearch US and JIR Europe. Promotion is not available to distribution partners or procurement platforms, such as Ariba and SciQuest.<\/li>\n<li>The discount code must be entered during the checkout process or at the time of order to receive the discount. For online orders, codes need to be redeemed in the comments section.<\/li>\n<li>The code can be used multiple times.<\/li>\n<li>The code is valid until December 31, 2025.<\/li>\n<li>This offer cannot be combined with or used in addition to any volume or institutional discount.<\/li>\n<\/ul>\n<a style=\"display:block;margin:1rem auto 10px;\" href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/AffiniPure-VHH-secondary-antibodies\"><img class=\"aligncenter wp-image-9850 size-full\" style=\"width: 100%;\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/VHH-affinipure-Q4-push_creatives_GIF_email_600x107_01.gif\" alt=\"\"><\/a>\n<table class=\"table blogLinks\">\n<thead>\n<tr>\n<th class=\"span6\">Learn more:<\/th>\n<th class=\"span6\">Do more:<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td class=\"span6\"><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/directandindirectwesternblotting\/\">Indirect and direct Western blotting<\/a><\/td>\n<td class=\"span6\"><a href=\"https:\/\/jacksonimmuno.com\/home\/exhibitions\">Exhibition Schedule<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\"><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/company-news\/meet-the-team-marketing\/\">Meet the Marketing Team<\/a><\/td>\n<td class=\"span6\"><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/western-blotting-guide\/\">Western blotting guide<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\"><a class=\"row-title\" href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/expansionmicroscopy\/\">An Introduction to Expansion Microscopy<\/a><\/td>\n<td class=\"span6\"><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/elisa-guide\/\">ELISA guide<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<script>\r\n\/\/ if (Intl.DateTimeFormat().resolvedOptions().timeZone.split('\/')[0] !== \"America\" && window.location.href.indexOf(\"utm_source=signature\") > -1) {\r\n\/\/     window.location.replace(\"https:\/\/www.jacksonimmuno.com\");\r\n\/\/ }\r\n\/\/ \r\n\tif (window.location.href.indexOf(\"utm_source=email-signature\") > -1) {\r\n\t\twindow.location.replace(\"https:\/\/www.jacksonimmuno.com\/technical\/products\/groups\/anti-gfp\/affinipure-vhh\");\r\n\t}\r\n<\/script>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Download PDF Green Fluorescent Protein (GFP) is a protein tag commonly used in fusion proteins as a reporter molecule. GFP has utility across numerous applications, including quantification of gene expression, protein localization within living organisms, characterization of protein interactions, and as a biosensor. The addition of an Anti-GFP antibody can often improve experimental performance and [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":3,"featured_media":11466,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"content-type":""},"categories":[19,20],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/11456"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=11456"}],"version-history":[{"count":18,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/11456\/revisions"}],"predecessor-version":[{"id":11496,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/11456\/revisions\/11496"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/11466"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=11456"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/categories?post=11456"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/tags?post=11456"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}