{"id":8922,"date":"2022-04-28T04:01:00","date_gmt":"2022-04-28T08:01:00","guid":{"rendered":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/?p=8922"},"modified":"2023-10-19T06:09:37","modified_gmt":"2023-10-19T10:09:37","slug":"nano-secondaries-test-drive-with-derek-sung","status":"publish","type":"post","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/company-news\/nano-secondaries-test-drive-with-derek-sung\/","title":{"rendered":"Subcellular imaging with JIR AffiniPure-VHH\u2122 Secondary Antibodies"},"content":{"rendered":"\n\n\n\t<div class=\"dkpdf-button-container\" style=\" text-align:right \">\n\n\t\t<a class=\"dkpdf-button\" href=\"\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/8922?pdf=8922\" target=\"_blank\"><span class=\"dkpdf-button-icon\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span> Download PDF<\/a>\n\n\t<\/div>\n\n\n\n\n\n<style>.entry p{font-size: 1rem;}.entry h3{color:#009fe3;margin-top:0}.entry h4{color:#009453;margin-top:1.5rem}.entry h5{color: #003a5c; font-size: 1.05rem;margin-top:1.5rem;}.entry figure{margin:32px auto;border:1px solid #ccc}.entry figure img{width:100%;display:block;margin:0 auto}.entry figcaption{font-size:.875rem;line-height:1.35rem;padding:10px;color:#222}.entry .blog-tbl{margin:1rem auto;caption-side:bottom}.entry .blog-tbl td,.entry .blog-tbl th{padding:10px 9px;border:1px solid #00172b;text-align:left}.entry .blog-tbl td{border-color:#003a5c}.entry .blog-tbl th{background-color:#00172b;color:#fff;border-left-color:#fff;border-right-color:#fff}.entry .blog-tbl th p{color:#fff}.entry .blog-tbl th:first-of-type{border-left-color:#00172b}.entry .blog-tbl th:last-of-type{border-right-color:#00172b}.entry .blog-tbl p{text-align:left;margin:0}.entry .box-note{border:2px solid #009453;padding:12px;margin:1rem 0}.entry .box-note p{margin:0;padding:0}.entry .styled-list{list-style-type:none}.entry .styled-list li{margin-top:1rem;line-height:1rem;font-size:1rem;}.entry .styled-list li::before{font-family:\"Font Awesome 5 Pro\";display:inline-block;content:\"\\f3c5\";-webkit-transform:rotate(-90deg);transform:rotate(-90deg);margin-left:-20px;margin-right:11px;font-size:.75rem;color:#ed7004;font-weight:600}.entry .styled-list ol li::before{display:none}.entry .styled-list li>ul li::before{font-weight:200}.entry .overview{width:-webkit-fit-content;width:-moz-fit-content;width:fit-content;padding:16px;margin:1rem auto;border:1px solid #eee}.entry .overview hr{margin-top:14px}.entry .overview-text{text-align:center;font-size:1rem;margin:0}<\/style>\n<p><script src=\"https:\/\/kit.fontawesome.com\/904923013f.js\" crossorigin=\"anonymous\"><\/script><\/p>\n<style>#attachment_8923{margin: 1rem auto;}#fig1 > div > div:first-of-type{flex-basis:24.4%}#fig1 > div > div:nth-of-type(2){flex-basis:40.5%}#fig1 > div > div:last-of-type{flex-basis:34.5%}#fig1 > div{display: flex;justify-content: space-between;}#fig1 > div > div:first-of-type::before{content: \"A\"} #fig1 > div > div:nth-of-type(2)::before{content: \"B\"}#fig1 > div > div:last-of-type::before{content: \"C\"} #fig1 > div > div::before{position:absolute;margin-top:-20px;font-weight:600;font-size:1rem;}#fig2{max-width:450px;}<\/style>\n<style>@media(max-width:530px){#fig1{max-width:270px}#fig1 > div {flex-direction:column}#fig1 > div > div {flex-basis:100%}#fig1 > div > div:nth-of-type(2){margin-top: 2rem;margin-bottom:2rem;}#fig1 > div > div:first-of-type{margin-top:2rem}#fig1 > div > div::before{margin-left:4px}}<\/style>\n<style>@media(min-width:980px){#fig1 > div > div:last-of-type{flex-basis:34.6%}}<\/style>\n<h2>We caught up with Derek Sung, Immunofluorescence guru, Ph.D. student, and the brains behind the outstanding Instagram identity &#8211; @immunofluorescence. Busy generating results for his Ph.D., he&#8217;s found time to test-drive our New AffiniPure-VHH\u2122 Secondary Antibodies. Read what he has been up to and find out how the AffiniPure-VHH\u2122 Secondary Antibodies behaved in his hands.<\/h2>\n<p><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/trophoblast-wide.png\" alt=\"\"><\/p>\n<h5>What have you been working on?<\/h5>\n<p><span style=\"font-weight: 400;\">\u201cI&#8217;m currently working on a few projects related to the placenta and how defects in the placenta influence organ development in the embryo, particularly the heart, brain, liver, and blood vessels. It&#8217;s a lot of fun because I can image all these different organs and see them at different stages of development.\u201d<\/span><\/p>\n<h5>What did you like about the (AffiniPure-VHH\u2122 Secondary) antibodies?<\/h5>\n<p><span style=\"font-weight: 400;\">\u201cI liked how there was no learning curve as someone who uses mainly goat and donkey secondary antibodies. The steps are almost exactly the same, and the signal is extremely high and long-lasting (the signal is still very high even three weeks later).\u201d<\/span><\/p>\n<h5>Were there any improvements you could see ?<\/h5>\n<p><span style=\"font-weight: 400;\">\u201cI did not notice any appreciable differences in resolution, but this is likely due to my imaging modality (widefield fluorescence). I think the size of the antibody will be most advantageous with super-resolution methods, such as SIM, TIRF, and STORM.\u201d<\/span><\/p>\n<div id=\"attachment_8923\" style=\"width: 310px\" class=\"wp-caption alignnone\"><img aria-describedby=\"caption-attachment-8923\" decoding=\"async\" loading=\"lazy\" class=\"wp-image-8923 size-medium\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/DS-Headshot-300x300.jpg\" alt=\"\" width=\"300\" height=\"300\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/DS-Headshot-300x300.jpg 300w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/DS-Headshot-1024x1024.jpg 1024w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/DS-Headshot-150x150.jpg 150w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/DS-Headshot-1536x1536.jpg 1536w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/DS-Headshot-45x45.jpg 45w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/DS-Headshot.jpg 1700w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><p id=\"caption-attachment-8923\" class=\"wp-caption-text\"><em>Derek Sung, MD\/PhD Student \u00b7 University of Pennsylvania School of Medicine<\/em><\/p><\/div>\n<p><strong>\u201cI&#8217;ve tested out the products and am happy to say that they worked GREAT in my hands.\u201d&nbsp;<\/strong><\/p>\n<h5>Are there any applications you can see them being useful for, or experiments you&#8217;d like to do with them?<\/h5>\n<p><span style=\"font-weight: 400;\">\u201cI think the nanobodies will be particularly useful in whole-mount immunofluorescence of cleared tissue. One of the major barriers to high-quality whole-mount immunofluorescence is the robust penetration of antibodies due to their size. Given that the nanobodies are ten times smaller than donkey or goat immunoglobulins, I think this will be really useful in staining entire tissues and organs.\u201d<\/span><\/p>\n<h5>Was blocking important?<\/h5>\n<p><span style=\"font-weight: 400;\">\u201cI only tested the products after blocking with 10% alpaca serum, but I had minimal background signal, so I would recommend blocking for optimal results.\u201d<\/span><\/p>\n<h5>Are there any other specificities or fluors you&#8217;d like us to add?<\/h5>\n<p><span style=\"font-weight: 400;\">\u201cOther common species for primary antibodies, such as goat and rat, would be great!\u201d<\/span><\/p>\n<h5>What are your plans for the future?<\/h5>\n<p><span style=\"font-weight: 400;\">\u201cI&#8217;m currently wrapping up my projects and preparing to defend my thesis in August. After that, I&#8217;ll return to medical school to finish my MD. I hope to one-day practice medicine while running a lab and continuing to study reproductive vascular biology.\u201d<\/span><\/p>\n<h5>\u201cI&#8217;ve been getting a lot of great messages regarding the antibodies! I think it&#8217;ll be a great product and resource for the community.\u201d<\/h5>\n<p><span style=\"font-weight: 400;\">The following images were made by Derek using JIR AffiniPure-VHH\u2122 Secondary Antibodies. &nbsp; \u201cThe first three images are of the lung, small intestine, and tongue\/palate all from a developing embryo. I stained these with E-cadherin (green) and alpha-smooth muscle actin (magenta). In each image, the E-cadherin outlines the epithelial cells really nicely and is surrounded by a ring of smooth muscle. You can see the developing airways in the lungs, the lining of the small intestine, and the layer of cells covering the tongue and palate.&#8221;&nbsp;<\/span><\/p>\n<figure id=\"fig1\">\n<div>\n<div><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Lung-sung-vhh-250x300.jpg\" alt=\"\"><\/div>\n<div><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Small-Intestine-No-DAPI-sml.jpg\" alt=\"\"><\/div>\n<div><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Tongue-Palate-No-DAPI-1-1-300x254.jpg\" alt=\"\"><\/div>\n<\/div><figcaption><strong>Figure 1.<\/strong> (A) Lung, (B) Small intestine, (C) Tongue and palate tissue, from embryos showing the developing structures. Tissues were stained with E-cadherin (green), showing the outlines of epithelial cells surrounded by a ring of smooth muscle as highlighted by staining for alpha-smooth muscle actin (magenta). These images used Alexa Fluor\u00ae488 VHH Fragment Alpaca Anti-Rabbit (<a href=\"https:\/\/www.jacksonimmuno.com\/catalog\/products\/611-544-215\">611-544-215<\/a>) and Alexa Fluor\u00ae647 VHH Fragment Alpaca Anti-Mouse(<a href=\"https:\/\/www.jacksonimmuno.com\/catalog\/products\/615-604-214\">615-604-214<\/a>) and blocked with 10% Alpaca serum (<a href=\"https:\/\/www.jacksonimmuno.com\/catalog\/products\/028-000-001\">028-000-001<\/a>), blue is DAPI nuclear stain.<\/figcaption><\/figure>\n<hr>\n<figure id=\"fig2\"><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/img\/static-pages\/jir-affinipure-vhh-trophoblast-1.jpg\" alt=\"\"><figcaption><strong>Figure 2.<\/strong> Trophoblasts (a type of cell from the placenta). I stained these with Cytokeratin 8 (blue), vinculin (green), and phalloidin (red). Cytokeratin is an intermediate filament, and vinculin is a type of focal adhesion. Phalloidin stains for F-actin. &#8220;<strong>I think this really highlights the ability to see subcellular structures using the secondaries.<\/strong>&#8221;<br \/>\nThese images used <a href=\"https:\/\/www.jacksonimmuno.com\/catalog\/products\/611-474-215\">611-474-215<\/a> (DyLight 405 VHH Fragment Alpaca Anti-Rabbit) and <a href=\"https:\/\/www.jacksonimmuno.com\/catalog\/products\/615-544-214\">615-544-214<\/a> (Alexa Fluor\u00ae 488 VHH Fragment Alpaca Anti-Mouse) and blocked with 10% Alpaca serum (<a href=\"https:\/\/www.jacksonimmuno.com\/catalog\/products\/028-000-001\">028-000-001<\/a>).<\/figcaption><\/figure>\n<table class=\"table blogLinks aligncenter\">\n<thead>\n<tr>\n<th class=\"span6\">Learn more:<\/th>\n<th class=\"span6\">Do more:<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td class=\"span6\"><a href=\"\/secondary-antibody-resource\/immuno-techniques\/colorimetric-western-blotting\/\">Colorimetric western blotting<\/a><\/td>\n<td class=\"span6\"><strong><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/conjugate-selection\/fluorophore-selection#spectra\">Spectra Viewer<\/a><\/strong><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\"><a href=\"\/secondary-antibody-resource\/immuno-techniques\/fluorescent-western-blotting\/\">Chemiluminescence western blotting<\/a><\/td>\n<td class=\"span6\"><a href=\"\/technical\/products\/groups\/signal-enhancement\">Antibodies for signal enhancement<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\" colspan=\"2\"><a href=\"\/secondary-antibody-resource\/immuno-techniques\/fluorescent-western-blotting\/\">Fluorescent western blotting<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Download PDF We caught up with Derek Sung, Immunofluorescence guru, Ph.D. student, and the brains behind the outstanding Instagram identity &#8211; @immunofluorescence. Busy generating results for his Ph.D., he&#8217;s found time to test-drive our New AffiniPure-VHH\u2122 Secondary Antibodies. Read what he has been up to and find out how the AffiniPure-VHH\u2122 Secondary Antibodies behaved in [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":3,"featured_media":8924,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"content-type":""},"categories":[19,20],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/8922"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=8922"}],"version-history":[{"count":41,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/8922\/revisions"}],"predecessor-version":[{"id":10596,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/8922\/revisions\/10596"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/8924"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=8922"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/categories?post=8922"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/tags?post=8922"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}