{"id":907,"date":"2022-08-01T07:33:57","date_gmt":"2022-08-01T11:33:57","guid":{"rendered":"https:\/\/jir-us-test.rwscott.co.uk\/secondary-antibody-resource\/?p=907"},"modified":"2022-08-01T07:34:01","modified_gmt":"2022-08-01T11:34:01","slug":"immunoglobulin-classes","status":"publish","type":"post","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/trending-topics\/immunoglobulin-classes\/","title":{"rendered":"Immunoglobulin classes"},"content":{"rendered":"\n\n\n\t<div class=\"dkpdf-button-container\" style=\" text-align:right \">\n\n\t\t<a class=\"dkpdf-button\" href=\"\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/907?pdf=907\" target=\"_blank\"><span class=\"dkpdf-button-icon\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span> Download PDF<\/a>\n\n\t<\/div>\n\n\n\n\n\n<style>.entry p, .entry ol{font-size: 1rem;}.entry h3{color:#009fe3;margin-top:0}.entry h4{color:#009453;margin-top:1.5rem}.entry h5{color: #003a5c; font-size: 1.05rem;margin-top:1.5rem;}.entry figure{margin:32px auto;border:1px solid #ccc}.entry figure img{width:100%;display:block;margin:0 auto}.entry figcaption{font-size:.875rem;line-height:1.35rem;padding:10px;color:#222}.entry .blog-tbl{margin:1rem auto;caption-side:bottom}.entry .blog-tbl td,.entry .blog-tbl th{padding:10px 9px;border:1px solid #00172b;text-align:left}.entry .blog-tbl td{border-color:#003a5c}.entry .blog-tbl th{background-color:#00172b;color:#fff;border-left-color:#fff;border-right-color:#fff}.entry .blog-tbl th p{color:#fff}.entry .blog-tbl th:first-of-type{border-left-color:#00172b}.entry .blog-tbl th:last-of-type{border-right-color:#00172b}.entry .blog-tbl p{text-align:left;margin:0}.entry .box-note{border:2px solid #009453;padding:12px;margin:1rem 0}.entry .box-note p{margin:0;padding:0}.entry .styled-list{list-style-type:none}.entry .styled-list li{margin-top:1rem;line-height:1rem;font-size:1rem;}.entry .styled-list li::before{font-family:\"Font Awesome 5 Pro\";display:inline-block;content:\"\\f3c5\";-webkit-transform:rotate(-90deg);transform:rotate(-90deg);margin-left:-20px;margin-right:11px;font-size:.75rem;color:#ed7004;font-weight:600}.entry .styled-list ol li::before{display:none}.entry .styled-list li>ul li::before{font-weight:200}.entry .overview{width:-webkit-fit-content;width:-moz-fit-content;width:fit-content;padding:16px;margin:1rem auto;border:1px solid #eee}.entry .overview hr{margin-top:14px}.entry .overview-text{text-align:center;font-size:1rem;margin:0}<\/style>\n<style>.entry figure{max-width: 500px;}.entry .ig-fig{max-width:300px;margin-top:0;}.entry .iga-fig{max-width: 350px;}<\/style>\n<p><script src=\"https:\/\/kit.fontawesome.com\/904923013f.js\" crossorigin=\"anonymous\"><\/script><\/p>\n<h3>Immunoglobulin Classes<\/h3>\n<h4>Introduction<\/h4>\n<p><span style=\"font-weight: 400;\">There are five classes of immunoglobulins, IgG, IgM, IgA, IgD, and IgE. These are distinguished by the type of heavy chain found in the molecule. IgG molecules have heavy chains known as gamma-chains (\u03b3); IgMs have mu-chains (\u03bc); IgAs have alpha-chains (\u03b1); IgEs have epsilon-chains (\u03b5), and IgDs have delta-chains (\u03b4).<\/span><\/p>\n<p><span style=\"font-weight: 400;\">Differences in heavy chain polypeptide sequence allow these immunoglobulins to function in different types of immune responses and at particular stages of the immune response. The polypeptide protein sequences responsible for these differences are found primarily in the Fc fragment.<\/span><\/p>\n<hr \/>\n<h4>IgA<\/h4>\n<figure class=\"ig-fig iga-fig\"><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/JIR14107-Jackson-Immuno-Research-UK-Immunoglobulins-illustrations_Key-1024x764.jpg\" alt=\"Key\" \/><br \/>\n<img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/JIR14107-Jackson-Immuno-Research-UK-Immunoglobulins-illustrations6-300x300.jpg\" alt=\"IgA\" \/><\/figure>\n<p><span style=\"font-weight: 400;\">Serum IgA is monomeric, whereas dimeric secretory IgA is found in all mucosal secretions. Dimer formation is facilitated by Fc domain \u201ctails\u201d and an interconnecting peptide (J chain), which facilitates the transport of IgA across the epithelial mucosa (transcytosis). Dimeric IgA binds with polymeric immunoglobulin receptor (pIgR) present on the epithelial cells. The complex is then internalized and transported via vesicles through the cytoplasm of the cell. Proteolytic cleavage of the pIgR releases the antibody to the epithelial mucosa. The extracellular domain of the pIgR is retained by the secreted dimeric IgA as the secretory component. IgA acts as a neutralizing antibody and is important in mucosal immunity, preventing invading pathogens from attaching and penetrating epithelial surfaces. IgA has two subclasses in humans.<\/span><\/p>\n<hr \/>\n<h4>IgD<\/h4>\n<figure class=\"ig-fig\"><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/JIR14107-Jackson-Immuno-Research-UK-Immunoglobulins-illustrations4-300x300.jpg\" alt=\"IgD\" \/><\/figure>\n<p><span style=\"font-weight: 400;\">IgD exists as a monomer and is found in low levels in serum. The role of IgD is uncertain, although its expression may correlate with the elimination of autoreactive B cells. IgD is co-expressed with IgM on B cell surfaces where it functions as antigen-specific BCR: the presence of IgD serves as a marker for the differentiation of B cells into their mature form. IgD does not bind complement. <\/span><span style=\"font-weight: 400;\">IgD may bind to basophils and mast cells, activating these cells to produce antimicrobial factors that function in the respiratory immune defense.<\/span><\/p>\n<hr \/>\n<h4>IgE<\/h4>\n<figure class=\"ig-fig\"><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/JIR14107-Jackson-Immuno-Research-UK-Immunoglobulins-illustrations5-300x300.jpg\" alt=\"IgE\" \/><\/figure>\n<p><span style=\"font-weight: 400;\">IgE is monomeric with an extra CH domain (CH4). IgE serum levels are low in healthy individuals: high IgE levels may indicate inflammatory disease, parasite burden, or allergy. When IgE binds to Fc receptors on basophils and mast cells, the activated cells release their granules; histamine, heparin, leukotrienes, and other compounds eliciting immediate hypersensitivity (allergic) reactions. IgE does not fix complement nor is it agglutinating, but it recruits eosinophils to clear parasitic infection.<\/span><\/p>\n<p><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/groups\/anti-human-secondary-antibodies\/ige\">Learn more about Anti Human-IgE antibodies<\/a><\/p>\n<hr \/>\n<h4>IgG<\/h4>\n<figure class=\"ig-fig\"><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/JIR14107-Jackson-Immuno-Research-UK-Immunoglobulins-illustrations2-300x300.jpg\" alt=\"IgG\" \/><\/figure>\n<p><span style=\"font-weight: 400;\">The Human IgG isotype consists of four subclasses named in order of their decreasing presence in adult human serum. Although the subtypes share &gt;90% sequence homology there is variation between them at the hinge and CH2 regions, as well as differences in the glycoforms present on the Fc. These subtle structural differences change the conformational flexibility and binding affinities of the subclasses, significantly impacting their biological function. The subclasses are named <\/span><span style=\"font-weight: 400;\">independently in each species: <\/span><span style=\"font-weight: 400;\">humans have four subclasses, horses seven, rabbits just one, and mice five. JIR provides secondary antibodies that are specific for the five mouse subclasses.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">IgG is important in the humoral immune response, providing protection from pathogens by neutralization, opsonization, and the complement pathway. IgG neutralizes viruses and bacteria by binding to pathogen surface proteins, preventing the pathogen from entering host cells and replicating. In opsonization, IgG binds pathogens to phagocytic cells (macrophages and neutrophils) via their Fc receptors (Fc\u03b3R), mediating phagocytosis and pathogen destruction.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">A final mode of protection comes via the complement pathway, in which IgG-coated pathogen is recognized by C1q protein. Binding of C1q to multiple Ig on the pathogen induces its conformational change which activates the classical complement cascade and subsequent clearance of the immunoglobulin-pathogen complex by phagocytosis and lysis.<\/span><\/p>\n<hr \/>\n<h4>IgM<\/h4>\n<figure class=\"ig-fig\"><img decoding=\"async\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/JIR14107-Jackson-Immuno-Research-UK-Immunoglobulins-illustrations-300x300.jpg\" alt=\"IgM\" \/><\/figure>\n<p><span style=\"font-weight: 400;\">IgM exists in 2 forms. Monomeric IgM is found on B cells, functioning as a B cell receptor (BCR). Pentamers (hexamers have also been found) that consist of 5 identical antibody units are found in circulation. These pentamers confer a high overall avidity from 10 binding sites, making IgM a potent complement activating antibody. IgM has an extra domain on the mu chain (CH4) and contains a J chain which is essential for the active transport of IgM through epithelial cells (see IgA). IgM does not cross the placenta, but is the first Ig to be made by the fetus and the first Ig to be made after immunization or exposure to antigen. Elevated levels of IgM may indicate recent infection or exposure to antigen.<\/span><\/p>\n<hr \/>\n<h4>Immunoglobulin (Light chain) isotypes<\/h4>\n<p><span style=\"font-weight: 400;\">Immunoglobulin type is also classified by the light chains present. The light chains may be either kappa (\u03ba), or one of 4 lambda (\u03bb) subtypes, and either light chain can be associated with any of the heavy chains. The ratio between \u03ba and \u03bb light chains varies between species, e.g. humans\u2019 ratio of \u03ba to \u03bb light chains is about 60:40, whereas the ratio in mice is 90:10.<\/span><\/p>\n<figure><img decoding=\"async\" loading=\"lazy\" width=\"512\" height=\"273\" class=\"size-full wp-image-8522\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/IgG-Fc-glycosylation.jpg\" alt=\"\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/IgG-Fc-glycosylation.jpg 512w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/IgG-Fc-glycosylation-300x160.jpg 300w\" sizes=\"(max-width: 512px) 100vw, 512px\" \/><figcaption>Figure 1: Ribbon diagram of an IgG showing the light chains in blue and the heavy chains in green. The sugar on the Fc is shown in purple.<\/figcaption><\/figure>\n<hr \/>\n<table class=\"table blogLinks aligncenter\">\n<thead>\n<tr>\n<th class=\"span6\">Learn more:<\/th>\n<th class=\"span6\">Do more:<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td class=\"span6\"><a href=\"\/secondary-antibody-resource\/immuno-techniques\/colorimetric-western-blotting\/\">Colorimetric western blotting<\/a><\/td>\n<td class=\"span6\"><strong><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/conjugate-selection\/fluorophore-selection#spectra\">Spectra Viewer<\/a><\/strong><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\"><a href=\"\/secondary-antibody-resource\/immuno-techniques\/fluorescent-western-blotting\/\">Chemiluminescence western blotting<\/a><\/td>\n<td class=\"span6\"><a href=\"\/technical\/products\/groups\/signal-enhancement\">Antibodies for signal enhancement<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\" colspan=\"2\"><a href=\"\/secondary-antibody-resource\/immuno-techniques\/fluorescent-western-blotting\/\">Fluorescent western blotting<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Download PDF Immunoglobulin Classes Introduction There are five classes of immunoglobulins, IgG, IgM, IgA, IgD, and IgE. These are distinguished by the type of heavy chain found in the molecule. IgG molecules have heavy chains known as gamma-chains (\u03b3); IgMs have mu-chains (\u03bc); IgAs have alpha-chains (\u03b1); IgEs have epsilon-chains (\u03b5), and IgDs have delta-chains [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":3,"featured_media":9060,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"content-type":""},"categories":[20],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/907"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=907"}],"version-history":[{"count":34,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/907\/revisions"}],"predecessor-version":[{"id":11376,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/907\/revisions\/11376"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/9060"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=907"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/categories?post=907"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/tags?post=907"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}