{"id":9858,"date":"2023-10-11T07:03:00","date_gmt":"2023-10-11T11:03:00","guid":{"rendered":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/?p=9858"},"modified":"2023-08-07T07:04:03","modified_gmt":"2023-08-07T11:04:03","slug":"tissue-clearing-techniques","status":"publish","type":"post","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/tissue-clearing-techniques\/","title":{"rendered":"Tissue Clearing Techniques"},"content":{"rendered":"\n\n\n\t<div class=\"dkpdf-button-container\" style=\" text-align:right \">\n\n\t\t<a class=\"dkpdf-button\" href=\"\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/9858?pdf=9858\" target=\"_blank\"><span class=\"dkpdf-button-icon\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span> Download PDF<\/a>\n\n\t<\/div>\n\n\n\n\n\n<p><style>.entry p, .entry ol{font-size: 1rem;}.entry h3{color:#009fe3;margin-top:0}.entry h4{color:#009453;margin-top:1.5rem}.entry h5{color: #003a5c; font-size: 1.05rem;margin-top:1.5rem;}.entry figure{margin:32px auto;border:1px solid #ccc}.entry figure img{width:100%;display:block;margin:0 auto}.entry figcaption{font-size:.875rem;line-height:1.35rem;padding:10px;color:#222}.entry .blog-tbl{margin:1rem auto;caption-side:bottom}.entry .blog-tbl td,.entry .blog-tbl th{padding:10px 9px;border:1px solid #00172b;text-align:left}.entry .blog-tbl td{border-color:#003a5c}.entry .blog-tbl th{background-color:#00172b;color:#fff;border-left-color:#fff;border-right-color:#fff}.entry .blog-tbl th p{color:#fff}.entry .blog-tbl th:first-of-type{border-left-color:#00172b}.entry .blog-tbl th:last-of-type{border-right-color:#00172b}.entry .blog-tbl p{text-align:left;margin:0}.entry .box-note{border:2px solid #009453;padding:12px;margin:1rem 0}.entry .box-note p{margin:0;padding:0}.entry .styled-list{list-style-type:none}.entry .styled-list li{margin-top:1rem;line-height:1rem;font-size:1rem;}.entry .styled-list li::before{font-family:\"Font Awesome 5 Pro\";display:inline-block;content:\"\\f3c5\";-webkit-transform:rotate(-90deg);transform:rotate(-90deg);margin-left:-20px;margin-right:11px;font-size:.75rem;color:#ed7004;font-weight:600}.entry .styled-list ol li::before{display:none}.entry .styled-list li>ul li::before{font-weight:200}.entry .overview{width:-webkit-fit-content;width:-moz-fit-content;width:fit-content;padding:16px;margin:1rem auto;border:1px solid #eee}.entry .overview hr{margin-top:14px}.entry .overview-text{text-align:center;font-size:1rem;margin:0}.entry .btn-sq{color:#fff;font-size:.9rem;font-weight:600;border:2px solid rgb(237, 112, 4);padding:7px 13px;background:rgb(237, 112, 4);cursor:pointer;text-align:center;line-height:1.5rem;margin:0 auto;}.entry .btn-sq:hover{text-decoration:none;color:rgb(237, 112, 4);background:#fff;}.entry .btn-container{display:flex;width:100%;margin:1.3rem 0;}.entry .btn-container .fa-solid, .entry .btn-container .fa-duotone{margin-right:10px;}@media(max-width: 768px){.entry .btn-sq{font-size:1.05rem;}}<\/style> <style>.entry .styled-list li::before{display:none} .entry .styled-list li{list-style-type:decimal;line-height:24px}.entry .styled-list li::marker{font-size:75%}<\/style><\/p>\n<p><script src=\"https:\/\/kit.fontawesome.com\/904923013f.js\" crossorigin=\"anonymous\"><\/script><\/p>\n<h2>The term \u2018tissue clearing\u2019 describes a collection of techniques used for making large, fixed biological specimens transparent. Tissue clearing enables volumetric (three-dimensional) imaging of sample types such as thick tissue sections, organoids, and intact organs using light microscopy, and can even be applied for studying whole organisms.<\/h2>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-9862 size-full\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/clarity.jpg\" alt=\"\" width=\"700\" height=\"248\" srcset=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/clarity.jpg 700w, https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/clarity-300x106.jpg 300w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><\/p>\n<h4>Tissue clearing principles<\/h4>\n<p>Biological samples have a high water content and contain multiple structures formed of proteins and\/or lipids. Each of these components has a different refractive index (the speed at which light travels through that component compared to the speed at which it travels through a vacuum), and it is the resultant heterogeneity within a sample which leads to a lack of transparency. Tissue clearing techniques aim to remove, replace, or alter some of the sample components to equilibrate the refractive index, thus rendering the biological specimen optically transparent.<\/p>\n<hr \/>\n<h4>Applications<\/h4>\n<p>Tissue clearing has become routine when imaging samples ranging from 100 \u03bcm to several centimeters in thickness. Compared to the established practice of slicing biological material into thin (~10 \u03bcm) sections for imaging, before piecing together the information obtained from adjacent slices, tissue clearing offers faster time to results and minimizes the risk of error.<\/p>\n<p>Additionally, tissue clearing is seeing increased use to enhance the capabilities of super-resolution microscopy. For example, by developing an optimized clearing agent for high-resolution fluorescence imaging (SeeDB2), researchers have been able to image tissue samples at a depth of &gt;100 \u03bcm<sup>1<\/sup>. This is reportedly an order of magnitude greater than was previously possible under standard mounting conditions.<\/p>\n<p>Importantly, introducing a tissue clearing step into standard immunostaining protocols represents a means of enabling high-resolution imaging to a depth of several hundred microns with regular confocal microscopes<sup>2<\/sup>. A growing number of tissue clearing techniques are available to researchers, each with different advantages and limitations.<\/p>\n<hr \/>\n<h4>Tissue clearing techniques<\/h4>\n<p>Tissue clearing techniques can broadly be categorized according to the type of chemistry they use. Each of the available approaches has evolved over time to address common challenges.<\/p>\n<p><strong>Organic solvents<\/strong> serve to dehydrate samples and remove lipids, resulting in a refractive index of around 1.55, which is thought to be comparable to that of proteinaceous structures<sup>2<\/sup>. The main advantage of using organic solvents is that they provide rapid clearing, making their use compatible with immunostaining protocols. However, dehydration prevents most fluorescent proteins from maintaining emissions.<\/p>\n<p>Methods designed to circumvent this problem include 3D imaging of solvent-cleared organs (3DISCO), in which the methanol dehydration step is replaced with incubation in tetrahydrofuran, and immunolabeling-enabled three-dimensional imaging of solvent-cleared organs (iDISCO), where methanol dehydration is followed by immunofluorescence with an antibody directed against the fluorescent protein in question<sup>3,4<\/sup>. These techniques have been shown to maintain fluorescent emissions for several days.<\/p>\n<p><strong>Aqueous-based tissue clearing <\/strong>involves submerging samples in a solution containing a high refractive index (1.44-1.52) molecule such as sucrose, fructose, or glycerol and leaving them to gradually become transparent<sup>5<\/sup>. Advantages of this approach include its simple protocol and the fact that (unlike using organic solvents) it enables direct lipid staining. However, aqueous-based tissue clearing can be prohibitively slow for larger samples, while the high viscosity of many concentrated sugar or glycerol solutions makes them difficult to work with.<\/p>\n<p>Within the past decade, a number of lower viscosity aqueous clearing solutions have been developed. These include FocusClear\u2122 and Histodenz\u2122, both of which come with a fairly high price tag, as well as lower cost options such as 2,2\u2032-thiodiethanol (TDE) and the FRUIT protocol. The latter is based on SeeDB2 (see above) and uses a cocktail of fructose and urea to synergistically clear samples including whole adult rabbit brains<sup>6<\/sup>.<\/p>\n<p><strong>Hyperhydration<\/strong> uses detergents to remove lipids from samples in conjunction with urea-mediated hydration, which lowers the refractive index to the 1.38\u20131.48 range<sup>2<\/sup>. By avoiding the use of organic solvents, hyperhydration maintain an aqueous environment for fluorescent proteins. However, its utility is limited by long clearing times, which make it impractical for integration into immunostaining protocols or processing large samples.<\/p>\n<p>The first hyperhydration technique to be described (Sca<em>l<\/em>e) has since been superseded by Sca<em>l<\/em>eS and other Sca<em>l<\/em>e family members<sup>7,8<\/sup>. These newer incarnations improve on the original method by being faster, avoiding tissue expansion, and preserving lipids. Another hyperhydration method, known as clear unobstructed brain imaging cocktails and computational analysis (CUBIC), includes an optional sucrose-based step to expedite clearing<sup>9<\/sup>. However, because CUBIC uses a high detergent concentration to maximize lipid removal, it suffers from significant protein loss<sup>5<\/sup>.<\/p>\n<p><strong>Hydrogel embedding <\/strong>stabilizes sample proteins by cross-linking them to a hydrogel. Any lipids are then removed using a detergent (or electrophoresis-based methods for faster lipid extraction) and the sample is submerged in a solution with a refractive index of 1.38-1.45. Although hydrogel embedding demonstrates excellent tissue clearing, detergent-based protocols are slow and electrophoresis-based methods can be difficult to implement<sup>2<\/sup>.<\/p>\n<p>CLARITY is the earliest example of hydrogel embedding and remains in use today<sup>10<\/sup>. Within the past decade the original method has been modified to yield methods termed passive CLARITY technique (PACT), perfusion-assisted agent release <em>in situ<\/em> (PARS), active CLARITY technique-pressure related efficient and stable transfer of macromolecules into organs (ACT-PRESTO), and system-wide control of interaction time and kinetics of chemicals (SWITCH), all of which employ different strategies to shorten experimental workflows<sup>2<\/sup>.<\/p>\n<hr \/>\n<h4>Factors to consider<\/h4>\n<p>Whenever there is a need for imaging fixed samples more than a few tens of microns thick, it is prudent to consider tissue clearing. Choosing between the available methods should involve careful evaluation of sample size and whether lipid staining is required, as well as include determining whether the clearing method should be compatible with immunostaining. For further guidance, speak with antibody and clearing agent manufacturers, who can often share relevant product reviews or publications.<\/p>\n<p>Jackson ImmunoResearch specializes in producing secondary antibodies for life science applications. Our portfolio encompasses a wide selection of products that are validated for common research applications, including immunofluorescence.<\/p>\n<hr \/>\n<h3>References<\/h3>\n<ol class=\"styled-list\">\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26972009\/\">Ke MT, Nakai Y, Fujimoto S, et al. Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent.\u00a0<i>Cell Rep<\/i>. 2016;14(11):2718-2732. doi:10.1016\/j.celrep.2016.02.057<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28082099\/\">Ariel P. A beginner&#8217;s guide to tissue clearing.\u00a0<i>Int J Biochem Cell Biol<\/i>. 2017;84:35-39. doi:10.1016\/j.biocel.2016.12.009<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23060243\/\">Ert\u00fcrk A, Becker K, J\u00e4hrling N, et al. Three-dimensional imaging of solvent-cleared organs using 3DISCO.\u00a0<i>Nat Protoc<\/i>. 2012;7(11):1983-1995. doi:10.1038\/nprot.2012.119<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25417164\/\">Renier N, Wu Z, Simon DJ, Yang J, Ariel P, Tessier-Lavigne M. iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging.\u00a0<i>Cell<\/i>. 2014;159(4):896-910. doi:10.1016\/j.cell.2014.10.010<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26186186\/\">Richardson DS, Lichtman JW. Clarifying Tissue Clearing.\u00a0<i>Cell<\/i>. 2015;162(2):246-257. doi:10.1016\/j.cell.2015.06.067<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25759641\/\">Hou B, Zhang D, Zhao S, et al. Scalable and DiI-compatible optical clearance of the mammalian brain.\u00a0<i>Front Neuroanat<\/i>. 2015;9:19. Published 2015 Feb 24. doi:10.3389\/fnana.2015.00019<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/21878933\/\">Hama H, Kurokawa H, Kawano H, et al. Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain.\u00a0<i>Nat Neurosci<\/i>. 2011;14(11):1481-1488. Published 2011 Aug 30. doi:10.1038\/nn.2928<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26368944\/\">Hama H, Hioki H, Namiki K, et al. ScaleS: an optical clearing palette for biological imaging.\u00a0<i>Nat Neurosci<\/i>. 2015;18(10):1518-1529. doi:10.1038\/nn.4107<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/24746791\/\">Susaki EA, Tainaka K, Perrin D, et al. Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis.\u00a0<i>Cell<\/i>. 2014;157(3):726-739. doi:10.1016\/j.cell.2014.03.042<\/a><\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23575631\/\">Chung K, Wallace J, Kim SY, et al. Structural and molecular interrogation of intact biological systems.\u00a0<i>Nature<\/i>. 2013;497(7449):332-337. doi:10.1038\/nature12107<\/a><\/li>\n<\/ol>\n<p><a href=\"https:\/\/www.jacksonimmuno.com\/technical\/products\/AffiniPure-VHH-secondary-antibodies\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-9850 size-full\" style=\"width: 100%;\" src=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/Nano-Secondaries-Advert-ML-GIFFING-4.gif\" alt=\"\" width=\"600\" height=\"100\" \/><\/a><\/p>\n<table class=\"table blogLinks\">\n<thead>\n<tr>\n<th class=\"span6\">Learn more:<\/th>\n<th class=\"span6\">Do more:<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td class=\"span6\"><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/directandindirectwesternblotting\/\">Indirect and direct Western blotting<\/a><\/td>\n<td class=\"span6\"><a href=\"https:\/\/jireurope.com\/home\/exhibitions\">Exhibition Schedule<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\"><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/chemiluminescent-western-blotting\/\">Chemiluminescence western blotting<\/a><\/td>\n<td class=\"span6\"><a href=\"https:\/\/jireurope.com\/home\/offers\/citeandwin\">Cite and Win<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"span6\"><a class=\"row-title\" href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-admin\/post.php?post=3642&amp;action=edit\" aria-label=\"\u201cAn Introduction to Expansion Microscopy\u201d (Edit)\">An Introduction to Expansion Microscopy<\/a><\/td>\n<td class=\"span6\"><a href=\"https:\/\/jireurope.com\/home\/offers\/previous-winners\">Past Competition Winners<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n<p><\/p>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Download PDF The term \u2018tissue clearing\u2019 describes a collection of techniques used for making large, fixed biological specimens transparent. Tissue clearing enables volumetric (three-dimensional) imaging of sample types such as thick tissue sections, organoids, and intact organs using light microscopy, and can even be applied for studying whole organisms. Tissue clearing principles Biological samples have [&hellip;]<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":11,"featured_media":9862,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"content-type":""},"categories":[3,16],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/9858"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=9858"}],"version-history":[{"count":18,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/9858\/revisions"}],"predecessor-version":[{"id":10513,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/9858\/revisions\/10513"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/9862"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=9858"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/categories?post=9858"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/tags?post=9858"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}