{"id":9963,"date":"2023-04-05T04:56:42","date_gmt":"2023-04-05T08:56:42","guid":{"rendered":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/?p=9963"},"modified":"2023-05-31T05:51:14","modified_gmt":"2023-05-31T09:51:14","slug":"elisa-guide","status":"publish","type":"post","link":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/elisa-guide\/","title":{"rendered":"NEW: 4 part ELISA Guide"},"content":{"rendered":"\n\n\n\t<div class=\"dkpdf-button-container\" style=\" text-align:right \">\n\n\t\t<a class=\"dkpdf-button\" href=\"\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/9963?pdf=9963\" target=\"_blank\"><span class=\"dkpdf-button-icon\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span> Download PDF<\/a>\n\n\t<\/div>\n\n\n\n\n\n<style>.part1-land-bg{background:url(\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-content\/uploads\/ELISA-1-1.jpg\") center\/cover 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980px){#page-content .landing-item h4{margin-top:8px;font-size:19px;line-height:1.7rem}.landing-item{height:auto}.landing-item h4{transform:translateY(0)}.landing-item p{position:static;opacity:1}.landing-item h5,.landing-item ul,.landing-item span{position:static;opacity:1}.landing-item span{margin-bottom:1rem}.landing-item .fa-angle-up{display:none;opacity:0}.landing-item .landing-img-background::before{background:rgba(0,0,0,.6)}.landing-item:hover ul{margin-bottom:1.3rem}.landing-item:hover span{margin-bottom:1rem}}@media(max-width: 768px){.landing-item span{display:block;margin-bottom:1rem}}@media(max-width: 550px){.landing-container{grid-template-columns:1fr 1fr;-moz-column-gap:0;column-gap:0;max-width:300px;margin:0 auto}.landing-odd .landing-item:last-of-type{grid-column:span 2}.landing-item{grid-column:span 2}}#page-content .landing-item a:hover{text-decoration:underline}#page-content .landing-item span a{color:#009fe3}#page-content .landing-item span a:hover{color:#008dca}#page-content .landing-item li a{color:#fff}#page-content .landing-item li a:hover{color:#ddd}.disabled-part{filter:grayscale(1)}<\/style>\r\n<style>#page-content .styled-list li::before {font-family: \"Font Awesome 6 Pro\";}<\/style>\r\n<style>#page-content{margin-top:1rem}<\/style>\r\n<script src=\"https:\/\/kit.fontawesome.com\/904923013f.js\" crossorigin=\"anonymous\"><\/script>\r\n<div id=\"page-content\">\r\n    <div class=\"landing-container\">\r\n        <div class=\"landing-item part1-land\">\r\n            <div class=\"landing-img-background part1-land-bg\">\r\n            <\/div>\r\n            <div class=\"landing-content\">\r\n                <h4>Part 1: Introduction to ELISA, Formats and Signal Amplification<\/h4>\r\n                <ul class=\"styled-list\">\r\n                    <li><a href=\"\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-1\/#elisa-formats\">ELISA Formats<\/a><\/li>\r\n                    <li><a href=\"\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-1\/#signal-amplification\">Signal Amplification<\/a><\/li>\r\n                <\/ul>\r\n                <i class=\"fal fa-angle-up\"><\/i>\r\n                <span><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-1\/\">Learn More<\/a><\/span>\r\n            <\/div>\r\n        <\/div>\r\n\r\n        <div class=\"landing-item part2-land\">\r\n            <div class=\"landing-img-background part2-land-bg\">\r\n            <\/div>\r\n            <div class=\"landing-content\">\r\n                <h4>Part 2: The ELISA Protocol<\/h4>\r\n                <ul class=\"styled-list\">\r\n                    <li><a href=\"\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-2\/#key-stages\">Key Stages<\/a><\/li>\r\n                    <li><a href=\"\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-2\/#workflow\">The ELISA Workflow<\/a><\/li>\r\n                <\/ul>\r\n                <i class=\"fal fa-angle-up\"><\/i>\r\n                <span><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-2\/\">Learn More<\/a><\/span>\r\n            <\/div>\r\n        <\/div>\r\n\r\n        <div class=\"landing-item part3-land\">\r\n            <div class=\"landing-img-background part3-land-bg\">\r\n            <\/div>\r\n            <div class=\"landing-content\">\r\n                <h4>Part 3: ELISA Optimization<\/h4>\r\n                <ul class=\"styled-list\">\r\n                    <li><a href=\"\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-3-elisa-optimization#optimization\">Optimization<\/a><\/li>\r\n                    <li><a href=\"\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-3-elisa-optimization#controls-standards\">Controls and Standards<\/a><\/li>\r\n                    <li><a href=\"\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-3-elisa-optimization#validation\">Validation<\/a><\/li>\r\n                <\/ul>\r\n                <i class=\"fal fa-angle-up\"><\/i>\r\n                <span><a href=\"\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-3-elisa-optimization\">Learn More<\/a><\/span>\r\n            <\/div>\r\n        <\/div>\r\n\r\n        <div class=\"landing-item part4-land\">\r\n            <div class=\"landing-img-background part4-land-bg\">\r\n            <\/div>\r\n            <div class=\"landing-content\">\r\n                <h4>Part 4: Troubleshooting<\/h4>\r\n                <ul class=\"styled-list\">\r\n                    <li><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-4-troubleshooting\/#no-signal-or-weak-signal\">No Signal or Weak Signal<\/a><\/li>\r\n                    <li><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-4-troubleshooting\/#high-background\">High Background<\/a><\/li>\r\n                    <li><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-4-troubleshooting\/#poor-reproducibility-between-plates\">Poor Reproducibility Between Plates<\/a><\/li>\r\n                    <li><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-4-troubleshooting\/#poor-reproducibility-between-runs\">Poor Reproducibility Between Runs<\/a><\/li>\r\n                    <li><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-4-troubleshooting\/#edge-effects-plate-drift\">Edge Effects \/ Plate Drift<\/a><\/li>\r\n                <\/ul>\r\n                <i class=\"fal fa-angle-up\"><\/i>\r\n                <span><a href=\"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/immuno-techniques\/elisa-guide-part-4-troubleshooting\/\">Learn More<\/a><\/span>\r\n            <\/div>\r\n        <\/div>\r\n    <\/div>\r\n<\/div>\n<!-- AddThis Advanced Settings generic via filter on the_content --><!-- AddThis Share Buttons generic via filter on the_content --><!-- AddThis Related Posts generic via filter on the_content -->","protected":false},"excerpt":{"rendered":"<p>Download PDF<!-- AddThis Advanced Settings generic via filter on get_the_excerpt --><!-- AddThis Share Buttons generic via filter on get_the_excerpt --><!-- AddThis Related Posts generic via filter on get_the_excerpt --><\/p>\n","protected":false},"author":10,"featured_media":9888,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"content-type":""},"categories":[19,3,16,20],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/9963"}],"collection":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/users\/10"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/comments?post=9963"}],"version-history":[{"count":3,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/9963\/revisions"}],"predecessor-version":[{"id":10324,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/posts\/9963\/revisions\/10324"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media\/9888"}],"wp:attachment":[{"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/media?parent=9963"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/categories?post=9963"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/wp-json\/wp\/v2\/tags?post=9963"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}