Distribution of Promyelocytic (PML)
Hep-2 cells expressing transiently the adenovirus-5 E4-ORF3 protein were analyzed by indirect immunofluorescence using a primary PML monoclonal Ab. Distinct PML-stained cell preparations were labeled with a highly pre-absorbed donkey anti-mouse secondary antibody conjugated to FITC (Jackson ImmunoResearch, code number 715-095-150). Fluorescence images were analyzed in confocal microscopy using a confocal laser scan fluorescence-inverted microscope (LSM 410, Zeiss). Confocal images represent single optical sections. Image analysis was subsequently entered into Photoshop where images were processed and magnified. PML and PML-associated macromolecular complexes have been implicated in mediating transcriptional regulation, suppressing cell cycle progression, inducing apoptosis or cellular senescence, and regulating viral propagation.
|Product used:||Product code:|
|Fluorescein (FITC)-AffiniPure Donkey Anti Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot)||715-095-150|
Dr. Vassilis Doucas, Collège-de France, C.N.R.S., Paris, France.