Immuno Techniques
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Vial fill size, protein concentration, and reconstitution volumes
We are sometimes asked about the discrepancy between the protein concentration recorded, the recommended volume …Read More »
Troubleshooting: Using Anti-Light Chain Antibodies
Anti-light chain antibodies are commonly used for Western blotting (WB) after Immunoprecipitation (IP) when detecting …Read More »
Western blotting guide: Part 7, Membrane Washing
Washing removes unbound or aggregated proteins present on the blot as well as unbound reagents …Read More »
Lateral Flow Immunoassay: Methodology, Applications, and Considerations for Use
Lateral flow immunoassay (LFIA) is a membrane-based technique for detecting specific analytes in complex samples. …Read More »Western blotting guide: Part 6, Secondary Antibodies
The secondary antibody detects the primary antibody, typically conjugated to a reporter molecule it enables …Read More »
Western blotting guide: Part 5, Primary Antibodies
Primary antibodies are used to detect the protein of interest. Part 5 of the Western …Read More »
Western blotting guide: Part 4, Membrane blocking
Blocking is essential to prevent non-specific interactions between the transferred proteins, the membrane and the …Read More »
Benefits of Lateral Flow Immunoassays for Point-Of-Care Testing
Lateral flow immunoassays (LFIA) are widely used for rapid detection of specific analytes in a …Read More »
Selecting Fluorophores for Antibody-based Research
Fluorophores are essential tools for scientific research. They are widely used for immunoassay techniques such …Read More »
Lateral flow tests for diagnostics
Lateral flow assays are one of the many types of immunoassays available for health monitoring. …Read More »Serological testing for diagnostics and disease surveillance
Serological tests enable disease surveillance from initial infection through to the development of immunity against …Read More »
Chromogenic Detection for Western Blot, IHC, and ELISA
Chromogenic substrates are used in colorimetric detection. They are simple and easy to use. Suitable …Read More »
Colorimetric Western blotting
Colorimetric detection is an economical and simple method for the detection of analyte when Western …Read More »
Chemiluminescent Western blotting
Chemiluminescent Western blotting is a highly sensitive protein detection method. The broad dynamic range allows …Read More »
Fluorescent Western blotting
Fluorescent Western blotting can offer many advantages to an already robust protein detection technique. Secondary …Read More »
Conjugates for Western blotting
3 methods of detection are available for Western blotting: colorimetric, chemiluminescent and fluorescent. Each detection …Read More »Direct and Indirect Western blotting
Western blotting is a robust technique employing antibodies to detect proteins immobilized on a blotting …Read More »
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