Purified IgG Fraction of Monoclonal
Mouse Anti-Biotin & Conjugates
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Our monoclonal Mouse Anti-Biotin antibody offers another alternative for amplification procedures, which can be used for
in situ hybridization, immunohistochemistry, and flow cytometry. Two examples of such amplification are shown below.
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These images are for viewing only, please do not reproduce. FISH of 75 kb low-copy genomic BAC (bacterial artificial
chromosome) clone of cotton (Gossypium hirsutum) to root-tip preparations from 26-chromosome relative,
G. arboreum.
Signal was detected with sequential applications of mouse anti-biotin and Cy3-conjugated goat anti-mouse antibodies.
Chromosomes were counterstained with DAPI. Photomicrographs were taken on Fujichrome 400, using an Olympus AX-70. |
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BAC FISH sites are visible in this interphase nucleus. Examples of applications include checking for chimerism among
BAC libraries, and assembly of integration maps (physical and genetic).
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Two pairs of signals, one per chromatid, are visible in this meristem mitotic, metaphase chromosome spread.
Images are from collaborative efforts of Dr. Rob Hanson, Dr. M. Nurul Islam-Faridi, Dr. David Stelly, and the BAC
Center, Texas A&M University. Photos submitted by Dr. Stelly, Texas A&M University. These images are for viewing
only; please do not reproduce. |
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