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"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."Janet Duerr, Ohio University
Whole IgG antibodies are suitable for the majority of immunodetection procedures.
F(ab')2 fragments are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors to Protein A or Protein G.
Fab fragments can be used to block endogenous immunoglobulins on cells, tissue, or other surfaces, and to block the exposed immunoglobulins in multiple labeling experiments using primary antibodies from the same species.
For distinguishing between two or more different subclasses of mouse IgG in multiple-labeling experiments, or for mouse IgG subclass determination.
Conjugates of streptavidin are recommended for use with Biotin‑SP‑conjugated antibodies and Biotin‑SP‑conjugated ChromPure proteins.
PAP Soluble Immune Complexes are prepared by the method of Sternberger et al.
For use in preparing affinity-purified antibodies or removing cross-reactive antibodies.
IgG fraction Monoclonal Mouse Anti-Biotin, Anti-Fluorescein, Anti-Digoxin, and Affinity-purified anti-horseradish peroxidase for signal enhancement.
Normal serums are recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc-receptor binding. Gamma Globulins are an inexpensive source of IgG.
Bovine serum albumin (BSA) is used extensively as a carrier protein to dilute antibodies and as a general protein blocking agent in immunoassays and immunodetection protocols.
ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals. Primarily for use as experimental controls.
Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide, and freeze-dried.