specializing in secondary antibodies and conjugates

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Secondary Antibodies

For VHH Discovery

Anti-Alpaca IgG, Subclass and VHH domain secondaries

Ideally time PBMC harvest

Enhance detection of VHH antibodies

Test for VHH expression and binding activity

Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Product Groups

Whole IgG Affinity-Purified Secondary Antibodies

Whole IgG antibodies are suitable for the majority of immunodetection procedures.

F(ab')₂ Fragment Affinity-Purified Secondary Antibodies

F(ab')₂ fragments are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors ot to Protein A or Protein G.

Monovalent Fab Fragment Affinity-Purified Secondary Antibodies

Fab fragments can be used to block endogenous immunoglobulins on cells, tissue, or other surfaces, and to block the exposed immunoglobulins in multiple labeling experiments using primary antibodies from the same species.

Anti-Mouse IgG Subclass Specific Antibodies

For distinguishing between two or more different subclasses of mouse IgG in multiple-labeling experiments, or for mouse IgG subclass determination.

Streptavidin

Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated antibodies and Biotin-SP-conjugated ChromPure proteins.

Peroxidase-Anti-Peroxidase (PAP) Soluble Immune Complexes

PAP Soluble Immune Complexes are prepared by the method of Sternberger et al.

Solid Phase Immunoadsorbent Gels

For use in preparing affinity-purified antibodies or removing cross-reactive antibodies.

Signal Enhancement

IgG fraction Monoclonal Mouse Anti-Biotin, Anti-Fluorescein, Anti-Digoxin, and Affinity-purified anti-horseradish peroxidase for signal enhancement.

Normal Serums and Gamma Globulins

Normal serums are recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc-receptor binding. Gamma Globulins are an inexpnsive source of IgG.

Bovine Serum Albumin

Bovine serum albumin (BSA) is used extensively as a carrier protein to dilute antibodies and as a general protein blocking agent in immunoassays and immunodetection protocols.

Purified Proteins from Normal Serums

ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals. Primarily for use as experimental controls.

Antisera to Immunoglobulins, Whole Serums, and Enzymes

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide, and freeze-dried.