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"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio University
Technical Service e-mail
Selection of fluorophores depends on:
The following seventeen fluorescent dye conjugates (Figure 1, table 1) are currently available from Jackson ImmunoResearch, and includes dyes in the Alexa Fluor®, Brilliant Violet™ and Cyanine ranges. They cover the most commonly used excitation sources and filter sets from blue to infrared emissions. Click on each fluorophore for more information.
Please note that we also have limited stock of DyLight™ 488 / 549 / 594 / 649 and Texas Red® conjugates.
Effective 4-color imaging with good color separation, good photostability, and high sensitivity is possible with combinations such as DyLight 405, Alexa Fluor® 488, Rhodamine Red-X, and Alexa Fluor® 647.
|Fluorophore||Excitation Peak||Emission Peak (nm)|
|Brilliant Violet 421™||407||421|
|Brilliant Violet 480™||436||478|
|Alexa Fluor® 488||493||519|
|R-Phycoerythrin R-PE||many, 488||580|
|Rhodamine Red-X, RRX||570||590|
|Alexa Fluor® 594||591||614|
|Allophycocyanin APC||many, 650||660|
|Alexa Fluor® 647||651||667|
|Peridinin-Chlorophyll-protein PerCP||many, 488||675|
|Alexa Fluor® 680||684||702|
|Alexa Fluor® 790||792||803|
Fluorescent probes or fluorophores (fluorescent dyes or proteins) are coupled to a secondary antibody or streptavidin to allow visualization of an analyte. Each fluorophore has its own spectral characteristics, with excitation and emission spectra particular to the molecule. Use the spectral viewer to build dye panels and compare the suitability of dyes for your application.