"Without question, I can always turn to Jackson ImmunoResearch secondary antibodies for ECL detection of any primary antibody! They produce great signal at low dilutions, last for years even through bad laboratory practice like repeatedly using the same tube every time, and come in a variety of species flavors."
W Thompson, OSHU
"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio University
Technical Service e-mail
Selection of fluorophores depends on:
The following seventeen fluorescent dye conjugates (Figure 1, table 1) are currently available from Jackson ImmunoResearch, and includes dyes in the Alexa Fluor®, Brilliant Violet™ and Cyanine ranges. They cover the most commonly used excitation sources and filter sets from blue to infrared emissions. Click on each fluorophore for more information.
Please note that we also have limited stock of DyLight™ 488 / 549 / 594 / 649 and Texas Red® conjugates.
Effective 4-color imaging with good color separation, good photostability, and high sensitivity is possible with combinations such as DyLight 405, Alexa Fluor® 488, Rhodamine Red-X, and Alexa Fluor® 647.
|Fluorophore||Excitation Peak||Emission Peak (nm)|
|Brilliant Violet 421™||407||421|
|Brilliant Violet 480™||436||478|
|Alexa Fluor® 488||493||519|
|R-Phycoerythrin R-PE||many, 488||580|
|Rhodamine Red-X, RRX||570||590|
|Alexa Fluor® 594||591||614|
|Allophycocyanin APC||many, 650||660|
|Alexa Fluor® 647||651||667|
|Peridinin-Chlorophyll-protein PerCP||many, 488||675|
|Alexa Fluor® 680||684||702|
|Alexa Fluor® 790||792||803|
Figure 1 illustrates the relative shape and position of each fluorophore in the peak region of its excitation and fluorescence emission following conjugation to antibodies. Quantitative comparisons should not be made since peak heights have been normalized. All spectra were obtained with an M-Series spectrophotometer system from Photon Technology International, Inc.