Technical Center
TechFAQ #4

What are the recommended storage conditions for
JIR's products?

Technical Service e-mail
tech@jacksonimmuno.com
  1. For Unconjugated Affinity-Purified (AffiniPure) Antibodies, IgG fraction Monoclonal Antibodies, Purified (ChromPure) Proteins from Normal Serums, and Gamma Globulins.

  2. For Normal Serums, Antisera, Peroxidase-Anti-Peroxidase (PAP) Soluble Immune Complexes.

  3. For Fluorophore (AMCA, Cy2, FITC, Cy3, TRITC, RRX, TR, Cy5) Conjugates, Biotin-SP Conjugates, and Horseradish Peroxidase Conjugates.

  4. For Alkaline Phosphatase-conjugated Affinity-Purified Antibodies and Purified Proteins.

  5. For Alkaline Phosphatase-conjugated Streptavidin.

  6. For Phycoerythrin Conjugates.

  7. For 12 and 18 nm Colloidal Gold-Antibody Complexes (EM Grade).

  8. For 6 nm Colloidal Gold-Antibody Complexes (EM Grade).

  9. For 4 nm Colloidal Gold-Antibody Complexes (LM Grade).

  10. For Solid-Phase Immunoadsorbent Gels.

  11. For Streptavidin and Bovine Serum Albumin.

For Unconjugated Affinity-Purified (AffiniPure) Antibodies, IgG fraction Monoclonal Antibodies, Purified (ChromPure) Proteins from Normal Serums, and Gamma Globulins:

 Physical State : Sterile-filtered liquid

Storage : Store at 2-8°C under sterile conditions. Prepare working dilution fresh each day. Expiration date: one year from date of receipt.

For Normal Serums, Antisera, Peroxidase-Anti-Peroxidase (PAP) Soluble Immune Complexes:

 Physical State : Freeze-dried powder

Reconstitution and Storage : Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration.

For Fluorophore (AMCA, Cy2, FITC, Cy3, TRITC, RRX, TR, Cy5) Conjugates, Biotin-SP Conjugates, and Horseradish Peroxidase Conjugates:     top of page

Physical State : Freeze-dried powder

 Reconstitution and Storage : Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol ( ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration.

For Alkaline Phosphatase-conjugated AffiniPure and Purified Proteins:     top of page

Physical State : Freeze-dried powder

 Reconstitution and Storage : Store freeze-dried powder at 2-8° C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol ( ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Expiration date: one year from date of rehydration.

For Alkaline Phosphatase-conjugated Streptavidin:     top of page

Physical State : Sterile-filtered liquid

 Storage : Product is stable for about 6 weeks at 2-8°C as an undiluted liquid - DO NOT FREEZE. Prepare working dilution fresh each day. For extended storage, add an equal volume of glycerol ( ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Expiration date: one year from the date of receipt.

For R-Phycoerythrin Conjugates:     top of page

Physical State : Freeze-dried powder

Reconstitution and Storage : Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Store at 2-8°C - DO NOT FREEZE. Prepare working dilution fresh each day. Expiration date: six months from date of rehydration.

For 12 and 18 nm Colloidal Gold-Antibody Complexes (EM Grade):     top of page

Physical State : Sterile-filtered liquid

Storage : Store at 2-8°C under sterile conditions. Prepare working dilution fresh each day. Expiration date: one year from date of receipt.

For 6 nm Colloidal Gold-Antibody Complexes (EM Grade):    top of page

Physical State : Sterile-filtered liquid

Storage : Store at 2-8°C under sterile conditions. Prepare working dilution fresh each day. Expiration date: six months from date of receipt.

For 4 nm Colloidal Gold-Antibody Complexes (LM Grade):      top of page

Physical State : Freeze-dried powder.

Reconstitution and Storage : Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. After rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Use an aliquot within one day of thawing. Expiration date: one year from date of rehydration.

For Solid-Phase Immunoadsorbent Gels:     top of page

Physical State : Agarose beads in buffer.

Storage : Store at 2-8°C. Expiration date: one year from date of receipt.


For Streptavidin and Bovine Serum Albumin:      top of page

Physical State : Freeze-dried powder.

Reconstitution and Storage : Store freeze-dried powder at 2-8°C. Weigh out desired amount and dissolve in desired buffer. Prepare working dilution fresh each day. Expiration date: one year from date of rehydration.

 

 
 
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