Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Rhodamine (TRITC) AffiniPure F(ab')₂ Fragment Goat Anti-Syrian Hamster IgG (H+L)

(min X Bov, Hrs, Hu, Ms, Rb, Rat Sr Prot)


Target: Syrian Hamster
Host: Goat
Antibody Format: F(ab')₂ Fragment
Specificity: IgG (H+L)
Minimal Cross Reactivity: Bovine, Horse, Human, Mouse, Rabbit, Rat Serum Proteins
Conjugate: Rhodamine (TRITC)
Product Category: F(ab')₂ Fragment Antibody Conjugates With Limited Stock
Clonality: Polyclonal
RRID: AB_2337453

Suitable for multiple labeling (see our Multiple Labeling guidance)
Discontinued - Limited Stock Available

Code: 107-026-142

Unit:
0.5 mg
Price:
$175.00



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Technical Info

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule syrian hamster IgG. It also reacts with the light chains of other syrian hamster immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse, human, mouse, rabbit and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

1:50 - 1:200 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Rhodamine (TRITC)

Amax: 550 Emax: 570nm

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

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