Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

Texas Red® AffiniPure™ Goat Anti-Human IgM, Fc fragment specific

(min X Bov Sr Prot)


Target: Human
Host: Goat
Antibody Format: Whole IgG
Specificity: IgM, Fc fragment specific
Minimal Cross Reactivity: Bovine Serum Proteins
Conjugate: Texas Red®
Product Category: Antibody Conjugates With Limited Stock
Clonality: Polyclonal
RRID: AB_2337646

Suitable for multiple labeling (see our Multiple Labeling guidance)
Discontinued - Limited Stock Available

Code: 109-075-129

Unit:
1.5 mg
Price:
$145.00


Download GHS/SDS

Technical Info
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of the human IgM heavy chain but not with human IgG, IgA, or the light chains of human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine serum proteins, but it may cross-react with IgM from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

1:50 - 1:200 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Texas Red®

Amax: 596 Emax: 620nm

This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
Jackson ImmunoResearch Laboratories, Inc. has trademark rights to AffiniPure™.

Texas Red is a trademark of Molecular Probes, Inc. and is registered with the U.S. Patent and Trademark Office.

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