Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Rabbit Anti-Sheep IgG (H+L)


Target: Sheep
Host: Rabbit
Specificity: IgG (H+L)
Conjugate: Unconjugated
Product Category: Antisera
RRID: AB_2339936

Warning: Bovine serum albumin (BSA) and dry milk may contain IgG. Secondary antibodies directed against alpaca, bovine, goat, horse, or sheep react with bovine IgG. Therefore, use of BSA or milk to block or dilute these antibodies may significantly increase background and/or reduce antibody titer. For blocking, normal serum (5% v/v) from the host species of the labeled secondary antibody is recommended.

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.

Based on immunoelectrophoresis, the antibody reacts with whole molecule sheep IgG. It also reacts with the light chains of other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

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