Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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AffiniPure Fab Fragment Rabbit Anti-Mouse IgG (H+L)


Target: Mouse
Host: Rabbit
Antibody Format: Fab Fragment
Specificity: IgG (H+L)
Conjugate: Unconjugated
Product Category: Fab Fragment Affinity‑Purified Antibodies
Clonality: Polyclonal
RRID: AB_2340047

Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.

Based on antigen-binding assay and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.
Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: None

Suggested Working Concentration or Dilution Range:

20-40 µg / ml

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

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