Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

Alexa Fluor® 680 VHH Fragment Alpaca Anti-Human IgG (H+L)

(min X Bov, Ms, Rb Sr Prot)


Target: Human
Host: Alpaca
Antibody Format: VHH Fragment
Specificity: IgG (H+L)
Minimal Cross Reactivity: Bovine, Mouse, Rabbit Serum Proteins
Conjugate: Alexa Fluor® 680
Product Category: Nano Secondary Antibodies

Code: 609-624-213

Unit:
0.1 mg
Price:
$295.00


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Technical Info
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, mouse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.

Nano Secondaries are polyclonal VHH Fragment antibodies (Nanobodies) produced in alpacas. 10x smaller than conventional whole IgG antibodies, the <15KDa VHH Fragment antibodies are perfect for imaging experiments where good penetration is necessary. Nano Secondary antibodies are cross-adsorbed for exquisite specificity against target species with minimal cross‑reactivity to other stated species, making them suitable for application in multiple labeling experiments.
Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

1:200 - 1:800 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Alexa Fluor® 680

Amax: 684 Emax: 702nm

Alexa Fluor® 680 and Alexa Fluor® 790 conjugates are used for very sensitive Western blots, ELISAs, and multiplexing arrays. Alexa Fluor® 680 conjugates are excited with a peak around 684 nm and fluoresce with a peak around 702 nm. Alexa Fluor® 790 conjugates are excited with a peak around 792 nm and fluoresce at a peak around 803 nm. They are the best choice for highly sensitive single or double labeling with fluorescence imaged in a LI-COR Odyssey® imager.

This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes. Product cannot be resold in any format without expressed authorization from Jackson ImmunoResearch Laboratories, Inc.

This product is provided under an agreement between Life Technologies Corporation and Jackson ImmunoResearch Laboratories, Inc, and the manufacture, use, sale or import of this product is sold pursuant to a license from Life Technologies Corporation for use of its fluorescent dye technology. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell or otherwise transfer this product or its components to any third party, or for any other commercial purposes. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 or outlicensing@lifetech.com.

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