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DyLight™ 405 AffiniPure-VHH® Fragment Alpaca Anti-Rabbit IgG (H+L)

(min X Bov, Hu, Ms Sr Prot)

Conjugate Options

Target: Rabbit
Host: Alpaca
Antibody Format: VHH Fragment
Specificity: IgG (H+L)
Minimal Cross Reactivity: Bovine, Human, Mouse Serum Proteins
Conjugate: DyLightâ„¢ 405
Product Category: AffiniPure-VHH® Secondary Antibodies
RRID: AB_2922864

This product is licensed for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes. Product cannot be resold in any format without expressed authorization from Jackson ImmunoResearch Laboratories, Inc.
Jackson ImmunoResearch Laboratories, Inc. has trademark rights to AffiniPure-VHH®.

Code: 611-474-215

Unit:
0.1 mg
Price:
$286.00

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Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, human and mouse serum proteins, but it may cross-react with immunoglobulins from other species.

AffiniPure-VHH® secondaries are polyclonal single-domain antibodies (nanobodies†) derived from hyper-immunized alpacas. 10x smaller than conventional whole IgG antibodies, <15KDa VHH fragments are perfect for imaging experiments where good penetration is necessary. AffiniPure-VHH® secondary antibodies are cross-adsorbed for exquisite specificity against target species with minimal cross‑reactivity to other stated species, making them suitable for application in multiple labeling experiments.

†Nanobody® is a trademark of ABLYNX N.V.
Usage
Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

1:200 - 1:800 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

DyLightâ„¢ 405

Amax: 400 Emax: 421nm

DyLight 405-conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use is limited to microscopes or flow cytometers equipped with a 405 nm laser and a 420 nm emission filter. Under these conditions, it is possible to perform effective 4-color imaging with good color separation, good photostability, and high sensitivity. The combination of DyLight 405, Alexa Fluor® 488, Rhodamine Red-X, and Alexa Fluor® 647 provides for maximum color separation. Another 4-color dye combination, which may be equally effective but has slightly less color separation, is DyLight 405, Alexa Fluor® 488, Cy3, and Alexa Fluor® 647. DyLight 405 conjugates are an excellent choice for blue-fluorescing secondary antibodies in multi-color labeling protocols.

Images & References

Trophoblasts staining highlighting subcellular imaging with JIR Nano Secondaries. Cytokeratin 8 (blue) (DyLight™ 405 VHH Fragment Anti-Rabbit (611‑474‑215)), vinculin (green) (Alexa Fluor® 488 VHH Fragment Anti-Mouse (615-544-214)), and phalloidin (F-actin) (red).

Cytokeratin is an intermediate filament and vinculin is a type of focal adhesion. Blocked with 10% Alpaca serum (028‑000‑001).

Picture credit: Derek Sung, MDPhD Student · University of Pennsylvania School of Medicine

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes. Product cannot be resold in any format without expressed authorization from Jackson ImmunoResearch Laboratories, Inc.


DyLightâ„¢ is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

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