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Rhodamine Red™-X (RRX) AffiniPure-VHH® Fragment Alpaca Anti-Mouse IgG (H+L)

(min X Bov, Hu, Rb Sr Prot)

Conjugate Options

Target: Mouse
Host: Alpaca
Antibody Format: VHH Fragment
Specificity: IgG (H+L)
Minimal Cross Reactivity: Bovine, Human, Rabbit Serum Proteins
Conjugate: Rhodamine Redâ„¢-X (RRX)
Product Category: AffiniPure-VHH® Secondary Antibodies
RRID: AB_2922872

This product is licensed for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes. Product cannot be resold in any format without expressed authorization from Jackson ImmunoResearch Laboratories, Inc.
Jackson ImmunoResearch Laboratories, Inc. has trademark rights to AffiniPure-VHH®.

Code: 615-294-214

Unit:
0.1 mg
Price:
$254.00

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Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, human and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.

AffiniPure-VHH® secondaries are polyclonal single-domain antibodies (nanobodies†) derived from hyper-immunized alpacas. 10x smaller than conventional whole IgG antibodies, <15KDa VHH fragments are perfect for imaging experiments where good penetration is necessary. AffiniPure-VHH® secondary antibodies are cross-adsorbed for exquisite specificity against target species with minimal cross‑reactivity to other stated species, making them suitable for application in multiple labeling experiments.

†Nanobody® is a trademark of ABLYNX N.V.
Usage
Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

1:100 - 1:400 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Rhodamine Redâ„¢-X (RRX)

Amax: 570 Emax: 590nm

RRX (Rhodamine Red-X) conjugates have a peak of excitation at 570 nm and a peak of emission at 590 nm. Although TRITC has been used traditionally with FITC for double labeling, better color separation is achieved by using RRX or Alexa Fluor® 594. Rhodamine Red-X is particularly useful for 3- and 4-color labeling with DyLight 405, Alexa Fluor® 488, and Alexa Fluor® 647 by using a confocal microscope equipped with a 405 nm laser and a krypton/argon laser. Fluorescence from RRX lies about midway between that of Alexa Fluor® 488 and Alexa Fluor® 647, and it shows little overlap with either dye. The krypton-argon laser emits lines at 488 nm, 568 nm, and 647 nm, which are optimal for exciting Alexa Fluor® 488, RRX, and Alexa Fluor® 647, respectively. By adding a 405 nm laser and a 420 nm emission filter, 4-color labeling is possible using DyLight 405-conjugated secondary antibodies from JIR (Figure 5). The separation between all four dyes is perfect for 4-color labeling, and all four dyes are very bright.

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes. Product cannot be resold in any format without expressed authorization from Jackson ImmunoResearch Laboratories, Inc.


Rhodamine Red-X is a trademark of Molecular Probes, Inc. Jackson ImmunoResearch is licensed by Molecular Probes, Inc. to manufacture and sell conjugates of Rhodamine Red-X reactive dye.

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