Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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R-Phycoerythrin AffiniPure F(ab')₂ Fragment Donkey Anti-Goat IgG (H+L)

(min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)


Target: Goat
Host: Donkey
Antibody Format: F(ab')₂ Fragment
Specificity: IgG (H+L)
Minimal Cross Reactivity: Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat Serum Proteins
Conjugate: R-Phycoerythrin
Product Category: Fluorescent Protein Conjugates (R-PE, APC, and PerCP)
Clonality: Polyclonal
RRID: AB_2340405

Suitable for multiple labeling (see our Multiple Labeling guidance)
Warning: Bovine serum albumin (BSA) and dry milk may contain IgG. Secondary antibodies directed against alpaca, bovine, goat, horse, or sheep react with bovine IgG. Therefore, use of BSA or milk to block or dilute these antibodies may significantly increase background and/or reduce antibody titer. For blocking, normal serum (5% v/v) from the host species of the labeled secondary antibody is recommended.

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule goat IgG. It also reacts with the light chains of other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, syrian hamster, horse, human, mouse, rabbit and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Store at 2-8°C – do not freeze. Prepare working dilution on day of use.
Expiration date: six months from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

1:50 - 1:200 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

R-Phycoerythrin

Amax: 488 Emax: 580nm

Phycoerythrin (R-PE) is among several kinds of light-harvesting phycobiliproteins found in red, blue-green, and cryptomonad algae. We offer R-PE, the form found in red macrophytic algae (seaweed). After phycobiliproteins are conjugated to secondary antibodies, there is little fluorescence quenching, which results in conjugates of high specific fluorescence compared with conventional fluorophore-antibody conjugates. R-PE can be excited by light over a wide range of the visible spectrum is highly water soluble, has a relatively low isoelectric point, and lacks potentially sticky carbohydrates.

It should be noted that the relatively high molecular weight of R-PE may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

R-Phycoerythrin is licensed under U.S. Patents 4,5220,110 and 4,542,104; E.P.O. Patent 0076695; and Canadian Patent 1179942.

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