Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

Find By Code Number

Product Filter

Spotlight on...

Multiple Labeling

With BV421™ & BV480™

  • Combine with AF488, RR-X, and AF647 for 5-color imaging
  • Compatible with commonly used filter sets
  • Switch nuclear stain from DAPI to DRAQ5 for additional labeling options

Learn More

Whole IgG Affinity-Purified Secondary Antibodies

"We only use secondary antibodies from Jackson ImmunoResearch whenever possible! Customer service is also excellent and the price point for their products is very competitive."

Elizabeth Soberg, University of Washington

Leave a Review

Meet JIR...

Exhibit Schedule

30th Aug - Life Science Exhibits, NYU School of Medicine - New York, NY

13-14th Sep - NIH Research Fall Festival - Bethesda, MD

21-26th Sep - NSH-Nat'l Soc For Histotechnology - St. Louis, MO

More info

JIREurope tweets...

Share your results...

Cite & Win!

Using our products?
About to publish?
Share your images with us!

Let us know!

Recommended Storage Conditions

Technical Service e-mail
tech@jacksonimmuno.com

TechFAQ #4

What are the recommended storage conditions for JIR's products?

  1. For Unconjugated Affinity-Purified (AffiniPure) Antibodies, IgG fraction Monoclonal Antibodies, Purified (ChromPure) Proteins from Normal Serums, and Gamma Globulins.
  2. For Normal Serums, Antisera, Peroxidase-Anti-Peroxidase (PAP) Soluble Immune Complexes.
  3. For Fluorophore (AMCA, Cy2, Alexa Fluor® 488, DyLight 488, FITC, DyLight 549, Cy3, TRITC, RRX, TR, Alexa Fluor® 594, DyLight 594, Alexa Fluor® 647, DyLight 649, Cy5, Alexa Fluor® 680, and Alexa Fluor® 790) Conjugates, Biotin-SP Conjugates, and Horseradish Peroxidase Conjugates.
  4. For Brilliant Violet™ Conjugates.
  5. For Alkaline Phosphatase-conjugated Affinity-Purified Antibodies and Purified Proteins.
  6. For Alkaline Phosphatase-conjugated Streptavidin.
  7. For R-Phycoerythrin, Allophycocyanin and PerCP Conjugates.
  8. For 18 nm Colloidal Gold-Antibody Complexes (EM Grade).
  9. For 6 and 12 nm Colloidal Gold-Antibody Complexes (EM Grade).
  10. For 4 nm Colloidal Gold-Antibody Complexes (LM Grade)
  11. For Solid-Phase Immunoadsorbent Gels.
  12. For Streptavidin and Bovine Serum Albumin.

For Unconjugated Affinity-Purified (AffiniPure) Antibodies, IgG fraction Monoclonal Antibodies, Purified (ChromPure) Proteins from Normal Serums, and Gamma Globulins:

Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution fresh each day. Expiration date: one year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For Normal Serums, Antisera, Peroxidase-Anti-Peroxidase (PAP) Soluble Immune Complexes:

Physical State: Freeze-dried solid

Reconstitution and Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For Fluorophore (AMCA, Cy2, Alexa Fluors®, DyLights™, FITC, Cy3, TRITC, RRX, TR, Cy5) Conjugates, Biotin-SP Conjugates, and Horseradish Peroxidase Conjugates:

Physical State: Freeze-dried solid

Reconstitution and Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For Brilliant Violet™ Conjugates:

Physical State: Freeze-dried solid

Reconstitution and Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use. Note: Store stained specimens at 4°C to preserve fluorescence.

For Alkaline Phosphatase-conjugated AffiniPure and Purified Proteins:

Physical State: Freeze-dried solid

Reconstitution and Storage: Store freeze-dried powder at 2-8° C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol ( ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For Alkaline Phosphatase-conjugated Streptavidin:

Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C. Prepare working dilution fresh each day. For extended storage, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: adding glycerol reduces the stated protein concentration and dilution range by one-half. Expiration date: one year from the date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For R-Phycoerythrin, Allophycocyanin and PerCP Conjugates:

Physical State: Freeze-dried solid

Reconstitution and Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Store at 2-8°C - DO NOT FREEZE. Prepare working dilution fresh each day. Expiration date: six months from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For 18 nm Colloidal Gold-Antibody Complexes (EM Grade):

Physical State: Sterile-filtered liquid

Storage: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Prepare working dilution fresh each day. Expiration date: one year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For 6 and 12 nm Colloidal Gold-Antibody Complexes (EM Grade):

Physical State: Sterile-filtered liquid

Storage: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Prepare working dilution fresh each day. Expiration date: six months from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For 4 nm Colloidal Gold-Antibody Complexes (LM Grade):

Physical State: Freeze-dried solid

Reconstitution and Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. After rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Use an aliquot within one day of thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For Solid-Phase Immunoadsorbent Gels:

Physical State: Agarose beads in buffer

Storage: Store at 2-8°C. Expiration date: one year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For Streptavidin

Physical State: Freeze-dried solid
Store freeze-dried powder at 2-8°C. Weigh out desired amount and dissolve in desired buffer. Centrifuge if not clear. Prepare working dilution fresh each day. For extended storage after rehydration, store under sterile conditions at 2-8°C or aliquot and freeze at -20°C or below. Expiration Date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.

For Bovine Serum Albumin

Physical State: Freeze-dried solid

Reconstitution and Storage
: Store freeze-dried powder at 2-8°C. Weigh out desired amount and dissolve in desired buffer. Prepare working dilution fresh each day.