Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Fluorescent Dyes

Brilliant Violet™
421 & 480

  • Combine with AF488, RR-X, & AF647 for 5-color imaging
  • Compatible with commonly used filter sets
  • Switch nuclear stain from DAPI to DRAQ5™ for additional labeling options

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Whole IgG Affinity-Purified Secondary Antibodies

"We only use secondary antibodies from Jackson ImmunoResearch whenever possible! Customer service is also excellent and the price point for their products is very competitive."

Elizabeth Soberg, University of Washington

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Exhibit Schedule

18th Jan - BRPF-Univ California, Davis Med Center - Sacramento, CA

19th Jan - BRPF-Univ California, San Francisco, Mission Bay - San Francisco, CA

20th Jan - BRPF-Univ California, San Francisco, Parnassus - San Francisco, CA

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Customer Reviews

What Do Scientists Say About Our Secondary Antibodies?

"We only use secondary antibodies from Jackson ImmunoResearch whenever possible! Customer service is also excellent and the price point for their products is very competitive."

Elizabeth Soberg, University of Washington

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Recommended Storage Conditions

Technical Service e-mail
tech@jacksonimmuno.com

TechFAQ #4

What are the recommended storage conditions for JIR's products?

  1. For Unconjugated Affinity-Purified (AffiniPure) Antibodies, IgG fraction Monoclonal Antibodies, Purified (ChromPure) Proteins from Normal Serums, and Gamma Globulins.
  2. For Normal Serums, Antisera, Peroxidase-Anti-Peroxidase (PAP) Soluble Immune Complexes.
  3. For Fluorophore (AMCA, Cy2, DyLight 488, FITC, DyLight 549, Cy3, TRITC, RRX, TR, DyLight 594, DyLight 649, and Cy5) Conjugates, Biotin-SP Conjugates, and Horseradish Peroxidase Conjugates.
  4. For Alkaline Phosphatase-conjugated Affinity-Purified Antibodies and Purified Proteins.
  5. For Alkaline Phosphatase-conjugated Streptavidin.
  6. For R-Phycoerythrin, Allophycocyanin and PerCP Conjugates.
  7. For 12 and 18 nm Colloidal Gold-Antibody Complexes (EM Grade).
  8. For 6 nm Colloidal Gold-Antibody Complexes (EM Grade).
  9. For 4 nm Colloidal Gold-Antibody Complexes (LM Grade)
  10. For Solid-Phase Immunoadsorbent Gels.
  11. For Streptavidin and Bovine Serum Albumin.

For Unconjugated Affinity-Purified (AffiniPure) Antibodies, IgG fraction Mono-clonal Antibodies, Purified (ChromPure) Proteins from Normal Serums, and Gamma Globulins:

Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution fresh each day. Expiration date: one year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For Normal Serums, Antisera, Peroxidase-Anti-Peroxidase (PAP) Soluble Immune Complexes:

Physical State: Freeze-dried powder

Reconstitution and Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For Fluorophore (AMCA, Cy2, FITC, Cy3, TRITC, RRX, TR, Cy5) Conjugates, Biotin-SP Conjugates, and Horseradish Peroxidase Conjugates:

Physical State: Freeze-dried powder

Reconstitution and Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol ( ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For Alkaline Phosphatase-conjugated AffiniPure and Purified Proteins:

Physical State: Freeze-dried powder

Reconstitution and Storage: Store freeze-dried powder at 2-8° C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol ( ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For Alkaline Phosphatase-conjugated Streptavidin:

Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C. Prepare working dilution fresh each day. For extended storage, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: adding glycerol reduces the stated protein concentration and dilution range by one-half. Expiration date: one year from the date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For R-Phycoerythrin, Allophycocyanin and PerCP Conjugates:

Physical State: Freeze-dried powder

Reconstitution and Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Store at 2-8°C - DO NOT FREEZE. Prepare working dilution fresh each day. Expiration date: six months from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For 12 and 18 nm Colloidal Gold-Antibody Complexes (EM Grade):

Physical State: Sterile-filtered liquid

Storage: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Prepare working dilution fresh each day. Expiration date: one year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For 6 nm Colloidal Gold-Antibody Complexes (EM Grade):

Physical State: Sterile-filtered liquid

Storage: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Prepare working dilution fresh each day. Expiration date: six months from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For 4 nm Colloidal Gold-Antibody Complexes (LM Grade):

Physical State: Freeze-dried powder.

Reconstitution and Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. After rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Use an aliquot within one day of thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For Solid-Phase Immunoadsorbent Gels:

Physical State: Agarose beads in buffer.

Storage: Store at 2-8°C. Expiration date: one year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

For Streptavidin and Bovine Serum Albumin

Physical State: Freeze-dried powder.

Reconstitution and Storage: Store freeze-dried powder at 2-8°C. Weigh out desired amount and dissolve in desired buffer. Prepare working dilution fresh each day.