Links to learn more about selection
and use of our products.

Choosing the right affinity-purified secondary
antibody for your application

Technical information on Fluorophores, Biotin-SP
(long-spacer), and Enzymes conjugated to our
antibodies and to other proteins


Links to general labeling guidelines
and protocols

Multiple labeling (ML) using labeled secondary
antibodies

Double labeling two primary antibodies from the
same host species

Silver Enhancement Protocol

The following information is intended to help those researchers who have experienced some
problems with the use of labeled secondary (or tertiary) antibodies.  Please submit questions to
us by e-mail or talk to us about your problems and we will try to answer them.  This section may
be revised from time-to-time as we receive more input from researchers who use our products.  
Click on the links below to learn more.

1. What are the common causes of background from secondary antibodies alone?
   
   
2. What are the possible causes of weak signals from labeled secondary antibodies assuming
   that antigens are present and primary antibodies are working?
   
   
3. What precaution should be considered before choosing a mounting medium for Cy2, Cy3,
   and Cy5 conjugates?
   
   
4. What are the recommended storage conditions for JIR's products.
   
   
5. What are the suggested dilutions factors to be used for Jackson ImmunoResearch products?
   
   
6. Why is DTAF-conjugated Streptavidin offered instead of FITC-conjugated Streptavidin?