Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Peroxidase ChromPure Chicken IgY (IgG), whole molecule


Host: Chicken
Product: IgY (IgG), whole molecule
Conjugate: Horseradish Peroxidase
Product Category: ChromPure™ Purified Proteins from Normal Serums
RRID: AB_2336968

IgY is the original designation for the IgG-like protein found in both serum and egg yolk.

Code: 003-030-003

Unit:
1.0 mg
Price:
$96.00



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Technical Info
ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals.
Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Purity: Based on immunoelectrophoresis at an antigen concentration of 20 mg/ml, the pattern of precipitation against goat anti-chicken whole serum is the same as that against goat anti-chicken IgY, Fc fragment specific.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)

Horseradish Peroxidase



Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

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