Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Purified Proteins

ChromPure™ Proteins (from Normal Serums) and Conjugates

ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals. The purified immunoglobulins in this section do not represent antibodies directed against known antigens.

Preparation

ChromPure proteins are prepared by a variety of methods, including ion-exchange, gel-filtration, hydrophobic, dye-ligand, metal-affinity, Protein A, and immunoaffinity chromatographies. Enzyme digestion is used to generate F(ab')2 (pepsin), and Fab and Fc (papain) fragments from highly purified whole molecules.

The digestion of an IgG using Papain and Pepsin digestion.

The digestion of IgG (approximate MW = 160 kDa) yields a number of different fragments. Papain digestion generates two monovalent Fab fragments and an Fc fragment, each about 50 kDa. Pepsin digestion degrades the Fc domain, leaving a divalent F(ab')2 fragment with approximate MW of 110 kDa.

Purity

No contaminating whole molecules or undesired fragments are observed at a protein concentration of 20 mg/ml when tested by immunoelectrophoresis against anti-whole serums, anti-immunoglobulins (class specific), or anti-fragment specific antisera.

Applications

ChromPure proteins are ideal for use as experimental controls (isotype controls).

Note: ChromPure proteins are not intended for use as immunogens to produce monospecific antibodies, nor should they be used as molecular weight markers.

Format

Unconjugated ChromPure proteins are supplied as sterile-filtered liquids without stabilizers or preservative. Conjugated ChromPure proteins are freeze-dried with stabilizers and a preservative, with the exception of peroxidase conjugates, which do not contain a preservative.


We also have a limited inventory of DyLight™ 488 / 549 / 594 / 647, Cy™2, Cy™5, and Texas Red® conjugates.

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