Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Biotin-SP (long spacer) ChromPure Donkey IgG, F(ab')2 fragment


Host: Donkey
Product: IgG, F(ab')2 fragment
Conjugate: Biotin-SP (long spacer)
Product Category: Purified Proteins from Normal Serum

Code: 017-060-006

Unit:
1.0 mg
Price:
$91.00



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Technical Info
ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals.
Physical State: Freeze-dried solid
Storage:

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


Purity: Based on immunoelectrophoresis at an antigen concentration of 20 mg/ml, the pattern of precipitation against rabbit anti-donkey whole serum is the same as that against goat anti-horse IgG, F(ab')2 fragment specific. No precipitin line was detected against rabbit anti-donkey IgG, Fc fragment specific.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Biotin-SP (long spacer)



Biotin-SP is our trade name for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

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