Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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R-Phycoerythrin AffiniPure Fab Fragment Goat Anti-Guinea Pig IgG, Fc fragment specific


Target: Guinea Pig
Host: Goat
Antibody Format: Fab Fragment
Specificity: IgG, Fc fragment specific
Conjugate: R-Phycoerythrin
Product Category: FabuLight™ - Affinity-Purified Fab Anti-Fc Fragment Specific Antibodies
Clonality: Polyclonal

FabuLights are suggested for labeling primary antibodies to be used in immunofluorescence or histochemical experiments. They are not recommended as a general blocking agent unless the γ or µ Fc region is to be targeted alone.

Code: 106-117-008

Unit:
0.5 ml
Price:
$237.00
Quantity:




Technical Info

Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of guinea pig IgG heavy chain but not with the Fab portion of guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Store at 2-8°C - DO NOT FREEZE. Prepare working dilution fresh each day.
Expiration date: six months from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Purity: The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

1:50 - 1:200 for sequential labeling applications.

To complex with primary antibody in solution, use 1:1 weight ratio of R-PE:primary antibody (approximate 3:1 molar ratio of Fab:primary antibody). Vortex and incubate for 30 minutes at room temperature prior to use. Titrate complex to optimal dilution for assay.

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

R-Phycoerythrin

Amax: 488 Emax: 580nm

Phycoerythrin (R-PE) is among several kinds of light-harvesting phycobiliproteins found in red, blue-green, and cryptomonad algae. We offer R-PE, the form found in red macrophytic algae (seaweed). After phycobiliproteins are conjugated to secondary antibodies, there is little fluorescence quenching, which results in conjugates of high specific fluorescence compared with conventional fluorophore-antibody conjugates. R-PE can be excited by light over a wide range of the visible spectrum is highly water soluble, has a relatively low isoelectric point, and lacks potentially sticky carbohydrates.

It should be noted that the relatively high molecular weight of R-PE may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

R-Phycoerythrin is licensed under U.S. Patents 4,5220,110 and 4,542,104; E.P.O. Patent 0076695; and Canadian Patent 1179942.