Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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4 nm Colloidal Gold AffiniPure Goat Anti-Human IgG (H+L) (LM Grade)

(min X Bov, Hrs, Ms Sr Prot)


Target: Human
Host: Goat
Antibody Format: Whole IgG
Specificity: IgG (H+L)
Minimal Cross Reactivity: Bovine, Horse, Mouse Serum Proteins
Conjugate: 4 nm Colloidal Gold
Product Category: Colloidal Gold-Antibody Complexes
Clonality: Polyclonal

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. After rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Use an aliquot within one day of thawing.
Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Borate - Sodium Phosphate, 0.15M NaCl, pH 8.5
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:
Histo-/Cyto-Chemistry:- 1:20-1:200

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

4 nm Colloidal Gold



LM Grade colloidal gold reagents are intended for light microscopy and immunoblotting. Since signal intensity is relatively independent of particle size when silver enhancement is used, we offer 4 nm particles because this size may penetrate tissues better than larger particles.

The 4 nm size may be used for electron microscopy in studies that require smaller particles since they are relatively uniform in size (coefficient of variation less than or equal to 15%), though small aggregates are not removed from this grade. Therefore, our 4 nm particles should only be used for electron microscopy with the
understanding that the presence of small aggregates may give misleading results.

A detailed protocol for silver enhancement is provided with all orders for LM Grade products. All reagents involved in the light-insensitive silver enhancement reaction can be prepared easily in the laboratory. However, those who wish to use commercially available silver enhancement kits can continue to do so. All LM Grade colloidal gold-protein complexes are freeze-dried in buffer with stabilizers and a preservative. After reconstitution, they may be frozen in aliquots for extended storage.

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.