Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

AffiniPure™ F(ab')₂ Fragment Goat Anti-Rat IgG, F(ab')₂ fragment specific

(min X Hu, Bov, Hrs Sr Prot)


Target: Rat
Host: Goat
Antibody Format: F(ab')₂ Fragment
Specificity: IgG, F(ab')₂ fragment specific
Minimal Cross Reactivity: Human, Bovine, Horse Serum Proteins
Conjugate: Unconjugated
Product Category: F(ab')₂ Fragment Affinity-Purified Antibodies
Clonality: Polyclonal
RRID: AB_2338107

Code: 112-006-072

Unit:
1.0 mg
Price:
$144.00


Download GHS/SDS

Technical Info
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab')2/Fab portion of rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against the Fc portion of rat IgG or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins, but it may cross-react with immunoglobulins from other species.

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.
Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: None

Suggested Working Concentration or Dilution Range:

10-20 µg / ml

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
Jackson ImmunoResearch Laboratories, Inc. has trademark rights to AffiniPure™.

go