Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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AffiniPure Fab Fragment Goat Anti-Rat IgM, µ chain specific


Target: Rat
Host: Goat
Antibody Format: Fab Fragment
Specificity: IgM, µ chain specific
Conjugate: Unconjugated
Product Category: FabuLight™ - Affinity-Purified Fc Specific Fab Fragments
Clonality: Polyclonal
RRID: AB_2632474

FabuLights are suggested for labeling primary antibodies to be used in immunofluorescence or histochemical experiments. They are not recommended as a general blocking agent unless the γ or µ Fc region is to be targeted alone.

Code: 112-007-020

Unit:
1.0 mg
Price:
$135.00



Technical Info

Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.

Based on antigen-binding assay and/or ELISA, the antibody reacts with the heavy chain of rat IgM but not with rat IgG or the light chains of rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with IgM from other species.
Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.
Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: None

Suggested Working Concentration or Dilution Range:

20-40 μg / ml

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

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