Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

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Alkaline Phosphatase AffiniPure Goat Anti-Rat IgG, light chain specific

(min X Bov, Gt, Hrs, Hu, Ms, Rb, Shp Ig)


Target: Rat
Host: Goat
Antibody Format: Whole IgG
Specificity: IgG, light chain specific
Minimal Cross Reactivity: Bovine, Goat, Horse, Human, Mouse, Rabbit, Sheep Immunoglobulins
Conjugate: Alkaline Phosphatase
Product Category: Anti‑Light Chain-Specific Antibodies for Western Blotting after IP
Clonality: Polyclonal

This antibody reacts primarily with kappa light chains. It is not suitable for detection of primary antibodies with lambda light chains.

Code: 112-055-175

Unit:
0.5 ml
Price:
$162.00



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Technical Info

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on rat IgG and with those common to other rat immunoglobulins. Reaction is primarily with kappa light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8° C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol ( ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original.
Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Tris-HCl, 0.25M NaCl, pH 8.0
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:
ELISA:- 1:5,000-1:50,000
Western Blot:- 1:5,000-1:50,000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Alkaline Phosphatase



Alkaline phosphatase (from calf intestine) conjugates are prepared by a modified method of Avremeas et al., Scand. J. Immunol. 1978. 8 (Supple. 7), 7. Resulting conjugates contain heterogeneous, high molecular weight complexes. They are sensitive reagents for solid-phase immunoassays such as ELISA and Western blotting. Although alkaline phosphatase conjugates are sometimes used for immunohistochemistry, penetration into whole mount tissues may be limited by their large sizes.

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.