Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.
Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG2b but not with the Fab portion of mouse immunoglobulins. No antibody was detected against mouse IgM or against non-immunoglobulin serum proteins. The antibody may cross-react with other mouse IgG subclasses or with immunoglobulins from other species.
Physical State: Sterile-filtered liquid
Storage: Store at 2-8°C under sterile conditions. Prepare working dilution fresh each day.
Expiration date: one year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Suggested Working Concentration or Dilution Range:
20-40 μg / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.