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Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Storage: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Prepare working dilution on day of use.
Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.
Colloidal gold reagents for transmission and scanning electron microscopy (EM Grade) are distinguished from other commercial preparations by careful separation of monomeric particles from small aggregates using ultracentrifugation in density gradients. The resulting monomeric colloidal gold-protein complexes are suspended in sterile-filtered buffer containing stabilizers and a preservative. All particle sizes in the EM Grade category may also be used for light microscopy and immunoblotting by those who prefer to use particles larger than 4 nm.
This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.