Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates

Find By Code Number

Product Filter

Brilliant Violet 480™ AffiniPure Goat Anti-Mouse IgG (H+L)

(min X Hu, Bov, Hrs, Rb, Sw Sr Prot)

Target: Mouse
Host: Goat
Antibody Format: Whole IgG
Specificity: IgG (H+L)
Minimal Cross Reactivity: Human, Bovine, Horse, Rabbit, Swine Serum Proteins
Conjugate: Brilliant Violet 480™
Product Category: Brilliant Violet™ Dye Conjugates for Multiple Labeling
Clonality: Polyclonal

Suitable for multiple labeling (see our Multiple Labeling guidance)

Code: 115-685-146

0.25 ml

Request GHS/SDS
Technical Info

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, horse, rabbit and swine serum proteins, but it may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solid

Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Note: Store stained specimens at 4°C to preserve fluorescence.

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Phosphate, 0.15M NaCl, 0.005% Tween 20, pH 7.2
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

1:50 - 1:200 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Brilliant Violet 480™

Amax: 436 Emax: 478nm

BD Brilliant™ Violet 480 (BV480) has an Excitation peak at 436 nm with maximal emission at 478 nm. Brilliant Violet polymer chains can be considered as a collection of optical segments, each with the ability to absorb light and emit fluorescence signal. This results in materials that have a bright fluorescence signal for better resolution and sensitivity.

If nuclear counterstaining is desired, 4-color antibody staining is possible using Brilliant Violet™ 421, Brilliant Violet™ 480, Alexa Fluor® 488 and Rhodamine Red™-X. If nuclear counterstaining is desired, 4-color antibody staining is possible using Brilliant Violet™ 421, Brilliant Violet™ 480, Alexa Fluor® 488 and Rhodamine Red™-X. Switching the nuclear stain from DAPI (emission in the blue region) to DRAQ5™ (which has red emission) frees the violet-blue region of the spectrum to accommodate the two Brilliant Violet dyes. DRAQ5’s excitation and emission profiles overlap those of Alexa Fluor 647.

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

This product is provided under an intellectual property license from Sirigen Inc., a Becton, Dickinson and Company affiliate. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Becton, Dickinson and Company, 10975 Torreyana Road, San Diego, California 92121 USA or BDBLicensing@bd.com.