"Without question, I can always turn to Jackson ImmunoResearch secondary antibodies for ECL detection of any primary antibody! They produce great signal at low dilutions, last for years even through bad laboratory practice like repeatedly using the same tube every time, and come in a variety of species flavors."
W Thompson, OSHU
14th Feb - Life Science Exhibits, Tufts University School of Medicine - Medford, MA
16th Feb - Life Science Exhibits, Harvard University Medical School - Boston, MA
23rd-25th Feb - ASCO-SITC Clinical Immuno-Oncology Symposium - Orlando, FL
"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio University
Add more colors to multiple labeling assays in the UV/Vis region, with Brilliant Violet 421™ and Brilliant Violet 480™ conjugated secondary antibodies. When combined with Alexa Fluor® 488, Rhodamine-Red™-X, and Alexa Fluor® 647 conjugates, effective 5-color fluorescent labeling is possible.
If nuclear counterstaining is desired, 4-color antibody staining is possible using Brilliant Violet 421, Brilliant Violet 480, Alexa Fluor® 488 and Rhodamine Red™-X. Switching the nuclear stain from DAPI (emission in the blue region) to DRAQ5™ (which has red emission) frees the violet-blue region of the spectrum to accommodate the two Brilliant Violet dyes. DRAQ5’s excitation and emission profiles overlap those of Alexa Fluor 647.
|Excitation Peak (nm)||Emission Peak (nm)|
|Brilliant Violet 421™||407||421|
|Brilliant Violet 480™||436||478|
We offer a range of secondary antibodies that are recommended for multiple labeling due to their minimal cross reactivity, ( See our multiple labeling guidelines for more information).
Available Target Specificities for Multiple Labeling (Designated ML* in product tables)
Excitation and emission curves for BV 421™, BV 480™, Alexa Fluor ® 488, Rhodamine Red™-X, and Alexa Fluor® 657.
DRAQ5 can be used as a nuclear stain in place of Alexa Fluor 647.
BD Brilliant Violet polymer chains can be considered as a collection of optical segments, each with the ability to absorb light and emit fluorescence signal. This results in materials that have a bright fluorescence signal for better resolution and sensitivity.
Light harvesting base polymers have multiple optical segments that can emit for fluorescence detection.
|Channel||Fluorophore||Excitation (nm)||Emission (nm)||Chroma Filter Set|
|Cyan||BV480™||436||478||49302 or 49001|
For more information on these filter sets, please go to the Chroma website.
|Fluorophore||Extinction co-efficient (M -1 cm-1)||Quantum Yield (ɸF)|
Brilliant Violet™ is a trademark of Sirigen Inc., a Becton, Dickinson and Company affiliate.