"Our laboratory has standardized the majority of our ELISA assays using JIR secondary antibodies. These antibodies have been proven to be extremely reliable, stable, and consistent. When I order products we receive them the next morning which is very convenient."
Mardi Reymann, University of MD Baltimore
1-5th Apr - AACR - American Association Cancer Research Annual Meeting - Washington, DC
22-26th Apr - EB - Experimental Biology - Chicago, IL
4th May - UMASS Medical School/ AbbVie Pharm, LSE - Worcester, MA
Add more colors to multiple labeling assays in the UV/Vis region, with Brilliant Violet 421™ and Brilliant Violet 480™ conjugated secondary antibodies. When combined with Alexa Fluor® 488, Rhodamine-Red™-X, and Alexa Fluor® 647 conjugates, effective 5-color fluorescent labeling is possible.
If nuclear counterstaining is desired, 4-color antibody staining is possible using Brilliant Violet 421, Brilliant Violet 480, Alexa Fluor® 488 and Rhodamine Red™-X. Switching the nuclear stain from DAPI (emission in the blue region) to DRAQ5™ (which has red emission) frees the violet-blue region of the spectrum to accommodate the two Brilliant Violet dyes. DRAQ5’s excitation and emission profiles overlap those of Alexa Fluor 647.
|Excitation Peak (nm)||Emission Peak (nm)|
|Brilliant Violet 421™||407||421|
|Brilliant Violet 480™||436||478|
We offer a range of secondary antibodies that are recommended for multiple labeling due to their minimal cross reactivity, ( See our multiple labeling guidelines for more information).
Available Target Specificities for Multiple Labeling (Designated ML* in product tables)
Excitation and emission curves for BV 421™, BV 480™, Alexa Fluor ® 488, Rhodamine Red™-X, and Alexa Fluor® 657.
DRAQ5 can be used as a nuclear stain in place of Alexa Fluor 647.
BD Brilliant Violet polymer chains can be considered as a collection of optical segments, each with the ability to absorb light and emit fluorescence signal. This results in materials that have a bright fluorescence signal for better resolution and sensitivity.
Light harvesting base polymers have multiple optical segments that can emit for fluorescence detection.
|Channel||Fluorophore||Excitation (nm)||Emission (nm)||Chroma Filter Set|
|Cyan||BV480™||436||478||49302 or 49001|
For more information on these filter sets, please go to the Chroma website.
|Fluorophore||Extinction co-efficient (M -1 cm-1)||Quantum Yield (ɸF)|
Brilliant Violet™ is a trademark of Sirigen Inc., a Becton, Dickinson and Company affiliate.